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373-Tomioka-CaImage/Fig2C_Ca_naiveWT_10

Author reviewed

Time-lapse calcium imaging of ASEL in naive WT C-elegans

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Project

373-Tomioka-CaImage

Dataset ID

Fig2C_Ca_naiveWT_10

Kind

Image data

Title

Time-lapse calcium imaging of ASEL in naive WT C-elegans

Description

Time-lapse calcium imaging of ASEL, a gustatory neuron, in naive wild type C-elegans. The autors used YC2.60 as a calcium indicator, and measured fluorescence intensity ratio of YFP to CFP. Left-half are CFP images, and Right half are YFP images. The glucose concentration was switched from 15 to 0 mM at 50 seconds and then returned to 15 mM at 100 seconds.

Folder Name

Fig2C_Ca_naiveWT_10

File Format

tif

File Size

128MB

File Separation Method

Figure Number

Fig2C, D

Dimensions

512x512x1x2x241

X Scale

0.4 micrometer

Y Scale

0.4 micrometer

Z Scale

T Scale

0.5 seconds

Channel

2 channel

Quantitative Data

Biosample

Description

A left-sided ASE gustatory neuron in wild type C. elegans.

Organism

Caenorhabditis elegans (NCBITaxon:6239)

Strain

JN2103 (http://api.wormbase.org/species/c_elegans/strain/WBStrain00058212#03--10)

Cell

neuron (WBbt:0003904)

Cell line

MeSH

Neurons (D009474)

Time-Lapse Imaging (D059008)

Fluorescence Resonance Energy Transfer (D031541)

Intrinsic Variable

Extrinsic Variable

gcy-7p::YC2.60 and lin-44p::mCherry were introduced as extrachromosomal arrays, glucose application

Ontology

Anatomical Entity

UBERON

Biological Process

Cellular Component

Molecular Function

MeSH Anatomy

Neurons (D009474)

Time-Lapse Imaging (D059008)

Fluorescence Resonance Energy Transfer (D031541)

MeSH BP

Neurons (D009474)

Time-Lapse Imaging (D059008)

Fluorescence Resonance Energy Transfer (D031541)

MeSH CC

Neurons (D009474)

Time-Lapse Imaging (D059008)

Fluorescence Resonance Energy Transfer (D031541)

MeSH MF

Neurons (D009474)

Time-Lapse Imaging (D059008)

Fluorescence Resonance Energy Transfer (D031541)

Genetics

Gene

Protein / Probe

-YC2.60

Method / Genotype

Microinjection for YC2.60 introduction / peEx2103[gcy-7p::YC2.60 (20 ng/ul); lin-44p::mCherry (20ng/ul)]

Treatments

Reagent / Compound

glucose

Concentration

0 or 15 millimolar

Fold Dilution

Imaging Method

fluorescence microscopy (FBbi:00000246)

time lapse microscopy (FBbi:00000249)

Detection Method

electron multiplying CCD (EMCCD)

Visualization Method

ECFP, Venus

Illumination Method

widefield illumination

Source of Contrast

Imaged Parameter

fluorescence emission

Sample Preparation Method

living tissue

MeSH

Neurons (D009474)

Time-Lapse Imaging (D059008)

Fluorescence Resonance Energy Transfer (D031541)

Instrument

Body

Leica DMI6000 B

Module

MAG BIOSYSTEMS DV2™ Dual-Channel, Simultaneous-Imaging System

Light Source

Leica EL6000

Detector

Hamamatsu ImagEM EM-CCD

Objective

Leica HCX PL APO 63x/1,40

Filter Set

Dichroic