Project
367-Tsuboi-DrosophilaWing
Title
The folding dynamics of Drosophila wing during pupal development
Description
Biological systems are inherently noisy; however, they produce highly stereotyped tissue morphology. Drosophila pupal wings show a highly stereotypic folding through uniform expansion and subsequent buckling of wing epithelium within a surrounding cuticle sac. The folding pattern produced by buckling is generally stochastic; it is thus unclear how buckling leads to stereotypic tissue folding of the wings. The authors found that the extracellular matrix (ECM) protein, Dumpy, guides the position and direction of buckling-induced folds. Dumpy anchors the wing epithelium to the overlying cuticle at specific tissue positions. Tissue-wide alterations of Dumpy deposition and degradation yielded different buckling patterns. In summary, they propose that spatiotemporal ECM remodeling shapes stereotyped tissue folding through dynamic interactions between the epithelium and its external structures.
Funding
This work was supported by JSPS KAKENHI 19J00764 (to A.T.), 21H05779 (to A.T.), 17H06386 (to K.F.), and 16H06280 “ABiS”; a grant from the NIPPON Genetics (to A.T.); The Keihanshin Consortium for Fostering the Next Generation of Global Leaders in Research (K- CONNEX) established by the program of Building of Consortia for the Development of Human Resources in Science and Technology, MEXT (to T.K.); and Japan Science and Technology Agency JPMJCR2121 (to K.F.).
Title
Z-series and time-lapse images of a Drosophila wing expressing mCD8::mCherry and Dpy::YFP in Np knockdown Drosophila pupa
Description
Z-series and time-lapse images of a Drosophila wing expressing mCD8::mCherry induced by sd-Gal4 and endogenous Dumpy::YFP in Notopleural (Np) knockdown Drosophila pupa.
Channel1; YFP, Channel2; mCherry
Description
A Drosophila pupa expressing mCD8::mCherry and Dpy::YFP. Notopleural was depleted from the wing.
Intrinsic variables
A transgenic Drosophila pupa expressing mCD8::mCherry and Dpy::YFP. Notopleural was depleted from the wing.
Gene ontology: Biological processes
Gene ontology: Cellular components
Gene ontology: Molecular functions
Protein names
SP460 (
Q9U3W7)
T-cell surface glycoprotein CD8 alpha chain (
P01731)
Dimensions
1024x1024x100x2x54
X scales
0.624 micrometer
Y scales
0.624 micrometer
Visualization methods
YFP
mCherry
Contrast enhancing methods
optical method
Resolution enhancing methods
Sample preparation methods
living tissue