Project
367-Tsuboi-DrosophilaWing
Title
The folding dynamics of Drosophila wing during pupal development
Description
Biological systems are inherently noisy; however, they produce highly stereotyped tissue morphology. Drosophila pupal wings show a highly stereotypic folding through uniform expansion and subsequent buckling of wing epithelium within a surrounding cuticle sac. The folding pattern produced by buckling is generally stochastic; it is thus unclear how buckling leads to stereotypic tissue folding of the wings. The authors found that the extracellular matrix (ECM) protein, Dumpy, guides the position and direction of buckling-induced folds. Dumpy anchors the wing epithelium to the overlying cuticle at specific tissue positions. Tissue-wide alterations of Dumpy deposition and degradation yielded different buckling patterns. In summary, they propose that spatiotemporal ECM remodeling shapes stereotyped tissue folding through dynamic interactions between the epithelium and its external structures.
Funding
This work was supported by JSPS KAKENHI 19J00764 (to A.T.), 21H05779 (to A.T.), 17H06386 (to K.F.), and 16H06280 “ABiS”; a grant from the NIPPON Genetics (to A.T.); The Keihanshin Consortium for Fostering the Next Generation of Global Leaders in Research (K- CONNEX) established by the program of Building of Consortia for the Development of Human Resources in Science and Technology, MEXT (to T.K.); and Japan Science and Technology Agency JPMJCR2121 (to K.F.).
Title
Z-series and time-lapse images of wings expressing Gap43::tdTomato and Dpy::YFP in control Drosophila pupa
Description
Z-series and time-lapse images of wings expressing Gap43::tdTomato and Dpy::YFP in control Drosophila pupa.
Channel1; YFP, Channel2; tdTomato
Description
A control Drosophila pupa expressing Gap43::tdTomato and Dpy::YFP in the wing. tdTomato was fused with N-terminal region of GAP43.
Strains
Dpy::YFP, Ubi-GAP43::tdTomato/+; rho^{ve-1}, vn^{1}/+
Intrinsic variables
A control Drosophila pupa expressing Gap43::tdTomato and Dpy::YFP in the wing
Gene ontology: Biological processes
Gene ontology: Cellular components
Gene ontology: Molecular functions
Dimensions
1024x1024x100x2x49
X scales
0.624 micrometer
Y scales
0.624 micrometer
Visualization methods
YFP
tdTomato
Contrast enhancing methods
optical method
Resolution enhancing methods
Sample preparation methods
living tissue