366-Ebata-TERTlocalize/FigS8_mVenus-TERT_2
Author reviewedTime-lapse images showing the translocation of mVenus-TERT in HeLa cells during apoptosis induced by oxidative stress
Project
Dataset ID
FigS8_mVenus-TERT_2
Kind
Image data
Title
Time-lapse images showing the translocation of mVenus-TERT in HeLa cells during apoptosis induced by oxidative stress
Description
Time-lapse images showing the translocation of mVenus-TERT (Telomerase reverse transcriptase) in HeLa cells during apoptosis induced by oxidative stress.This dataset is composed of 8 series of datasets; series1-series8, each of which is time-lapse image of 5 x 5 tiling data. Channel2; bright field, Channel4; mVenus, Channel5; MitoTracker Deep Red FM, Channe6; SYTOX Blue. The 8 series consist of 4 genotypes, and 2 experimental conditions.
Genotypes:
Series1-Series2; without transfection,
Series3-Series4; mVenus-TERT transfection,
Series5-Series6; mVenus-mutant TERT (R3ER6E) transfection,
Series7-Series8 mVenus-mutant TERT (Y707F) transfection.
Experimental conditions:
Series1, 3, 5, 7; without oxidative stress,
Series2, 4, 6, 8; with oxidative stress.
Folder Name
FigS8_mVenus-TERT_2
File Format
.nd2
File Size
102GB
File Separation Method
-
Figure Number
FigS8
Dimensions
5080x5080x1x4x41
X Scale
0.216 micrometer
Y Scale
0.216 micrometer
Z Scale
-
T Scale
1 hour
Channel
4 channel
Quantitative Data
-
Paper(s)
Biosample
Description
HeLa cells stabely expressing TERT (Telomerase reverse transcriptase) or mutant TERT (R3E/R6E or Y707F). All the wild type and mutant TERTs were tagged with mVenus.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
-
Cell line
HeLa cell (CLO:0003684)
MeSH
Intrinsic Variable
HeLa cells stabely expressing TERT (Telomerase reverse transcriptase) or mutant TERT (R3E/R6E or Y707F). All the wild type and mutant TERTs were tagged with mVenus.
Extrinsic Variable
SYTOX Blue application, which stains dead cells. MitoTracker Deep Red FM application, which stains mitochondria. Sodium percarbonate pretreatment as oxidative stress.
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
regulation of apoptotic process (GO:0042981)
Cellular Component
mitochondrion (GO:0005739)
nucleus (GO:0005634)
Molecular Function
telomerase activity (GO:0003720)
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
TERT
Protein / Probe
Telomerase reverse transcriptase
Method / Genotype
transfection
Gene
-
Protein / Probe
-SYTOX Blue
Method / Genotype
-
Gene
TERT
Protein / Probe
Telomerase reverse transcriptase / MitoTracker Deep Red FM
Method / Genotype
transfection
Treatments
Reagent / Compound
Sodium percarbonate
Concentration
267 micromolar
Fold Dilution
-
Imaging Method
Imaging Method
fluorescence microscopy (FBbi:00000246)
time lapse microscopy (FBbi:00000249)
Detection Method
wide-field detection
Visualization Method
mVenus, MitoTracker Deep Red FM, SYTOX Blue
Illumination Method
widefield illumination
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
living tissue
MeSH
Instrument
Body
inverted microscope (Ti, Nikon)
Module
-
Light Source
X-Cite XLED1, Lumen Dynaics
Detector
ImagEM X2-1K EM-CCD, Hamamatsu
Objective
Plan Apo Lambda 40×/0.95, Nikon
Filter Set
[CFP-2432C (for SYTOX-Blue or Cellstain Hoechst 33342; Semrock, Rochester, NY, USA), GFP-3035D (for YO-PRO-1; Semrock), LF514-B (for mVenus; Semrock), TRITC-A-Basic (for SYTOX-Orange; Semrock), and Cy5-4040C (for MitoTracker Deep Red FM; Semrock)].
Dichroic
-