The time-lapse NIR autofluorescence imaging of nuclear dynamics in Roquette root hair cells
Description
The nuclei were observed to transform and migrate within roquette root hair cells of Eruca vesicaria subsp. Sativa. Time-lapse NIR autofluorescence images of nuclear dynamics in Roquette root hair cells were obtained. The NIR autofluorescence imaging approach has no need for a fluorescent protein reporter and can reduce the genetic manipulation time of introducing encoded nuclear markers into mutant plants to improve the efficiency of monitoring the cell nuclei movement in root.
confocal microscopy (FBbi_00000251) fluorescence microscopy (FBbi_00000246) time lapse microscopy (FBbi_00000249)
X scale
0.312 micrometer
Y scale
0.312 micrometer
Z scale
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T scale
20.26 sec
Image Acquisition
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Objective model
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Summary of Methods
Yoshinari A, Isoda R, Yagi N, Sato Y, Lindeboom JJ, Ehrhardt DW, Frommer WB, Nakamura M. Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei. Plant J. 2024 Jun;118(5):1699-1712.
Related paper(s)
Contact
Akira Yoshinari, Masayoshi Nakamura
, Nagoya University, Nagoya University
, Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules
, Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules