Detail of MovS4_NIR_Roquette_RootTip_nuclei


Project
SSBD:Repository
Title
The time-lapse NIR autofluorescence imaging of nuclear dynamics in Roquette root tip cells
Description
The nuclei were observed to transform and migrate within roquette root tips cells of Eruca vesicaria subsp. Sativa. Time-lapse NIR autofluorescence images of nuclear dynamics in Roquette root tip cells were obtained. The NIR autofluorescence imaging approach has no need for a fluorescent protein reporter and can reduce the genetic manipulation time of introducing encoded nuclear markers into mutant plants to improve the efficiency of monitoring the cell nuclei movement in root.
Release, Updated
2026-06-09
License
CC BY 4.0
Kind
Image data
File Formats
czi
Data size
159.4 MB

Organism
Eruca vesicaria subsp. Sativa ( NCBI:txid29727 )
Strain(s)
-
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
nuclear migration
Cellular Component (CC)
nucleus
Biological Imaging Method
confocal microscopy (FBbi_00000251) fluorescence microscopy (FBbi_00000246) time lapse microscopy (FBbi_00000249)
X scale
0.624 micrometer
Y scale
0.624 micrometer
Z scale
-
T scale
20.26 sec

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
Yoshinari A, Isoda R, Yagi N, Sato Y, Lindeboom JJ, Ehrhardt DW, Frommer WB, Nakamura M. Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei. Plant J. 2024 Jun;118(5):1699-1712.
Related paper(s)

Contact
Akira Yoshinari, Masayoshi Nakamura , Nagoya University, Nagoya University , Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules , Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules
Contributors

OMERO Dataset
OMERO Project
Source