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Fig.1
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Fig.1B, 1C dextran injection for ERK FRET embryo Olympus FV1000 UPlanSApo 30 x NA1.05 silicon Zoom x1
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Figure data (edited)
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Fig.1D-1F dextran injection for ERK FRET embryo Olympus FV1000 UPlanSApo 60 x NA1.30 silicon Zoom x1
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Figure data (edited)
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Fig.2
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Fig.2A Control Par6-GFP his-RFP Olympus FV1000 UPlanSApo 30 x NA1.05 silicon Zoom x1
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Figure data (edited)
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raw data (Oib format)
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Fig.2B egfr[f24] Par6-GFP his-RFP Olympus FV1000 UPlanSApo 30 x NA1.05 silicon Zoom x1
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Figure data (edited)
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Fig.2D Control Par6-GFP his-RFP Olympus FV1000 PlanApo N 60 x NA1.42 oil Zoom x1
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Figure data (edited)
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Fig.2E egfr[f24] Par6-GFP his-RFP Olympus FV1000 PlanApo N 60 x NA1.42 oil Zoom x1
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Fig.3
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Fig.3A Control Ch1_DAPI_Ch2_cDcp1_Ch3_b-gal_Ch4_Dlg Olympus FV1000 UPLanSApO 60 x water NA 1.2 Zoom x1
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Figure data (edited)
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Fig.3B Df(3L)H99 Ch1_DAPI_Ch2_cDcp1_Ch3_b-gal_Ch4_Dlg Olympus FV1000 UPLanSApO 60 x water NA 1.2 Zoom x1
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Fig.3C egfr[f24] Ch1_DAPI_Ch2_cDcp1_Ch3_b-gal_Ch4_Dlg Olympus FV1000 UPLanSApO 60 x water NA 1.2 Zoom x1
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Figure data (edited)
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Fig.3D egfr[f24], Df(3L)H99 Ch1_DAPI_Ch2_cDcp1_Ch3_b-gal_Ch4_Dlg Olympus FV1000 UPLanSApO 60 x water NA 1.2 Zoom x1
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Figure data (edited)
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Fig.3E egfr[f24] Ch1_DAPI_Ch2_cDcp1_Ch3_b-gal_Ch4_Dlg Olympus FV1000 UPLanSApO 60 x water NA 1.2 Zoom x2
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Fig.3F egfr[f24] Ch1_DAPI_Ch2_cDcp1_Ch3_b-gal_Ch4_Dlg Olympus FV1000 UPLanSApO 60 x water NA 1.2 Zoom x2
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Figure data (edited)
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Fig.3G egfr[f24] Par6-GFP his-RFP Olympus FV1000 PlanApo N 60 x NA1.42 oil Zoom x2
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Fig.4
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Fig.4B Control Ch1_DAPI_Ch2_cDcp1_Ch3_b-gal_Ch4_Dlg Olympus FV1000 UPLanSApO 20 x NA 0.7 Zoom x1.5
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Figure data (edited)
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Fig.4C egfr[f24] Ch1_DAPI_Ch2_cDcp1_Ch3_b-gal_Ch4_Dlg Olympus FV1000 UPLanSApO 20 x NA 0.7 Zoom x1.5
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Fig.4D egfr[f24] Ch1_DAPI_Ch2_cDcp1_Ch3_b-gal_Ch4_Dlg Olympus FV1000 UPLanSApO 20 x NA 0.7 Zoom x1.5
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Fig.4E egfr[f24] Ch1_DAPI_Ch2_cDcp1_Ch3_b-gal_Ch4_Dlg Olympus FV1000 UPLanSApO 20 x NA 0.7 Zoom x1.5
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Fig.4F egfr[f24] Par6-GFP his-RFP Olympus FV1000 PlanApo N 60 x NA1.42 oil Zoom x2
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Fig.4G egfr[f24] Par6-GFP his-RFP Olympus FV1000 PlanApo N 60 x NA1.42 oil Zoom x2
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Fig.5
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Fig.5E Actin-Gal4 UAS-Apoliner clutured Olympus FV1000 PlanApo N 60 x NA1.42 oil Zoom x2
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Fig.5F egfr[f24] Par6-GFP his-RFP Olympus FV1000 PlanApo N 60 x NA1.42 oil Zoom x1
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Fig.5G egfr[f24] Par6-GFP his-RFP Olympus FV1000 PlanApo N 60 x NA1.42 oil Zoom x2
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Fig.6
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Fig.6A HS-GFP his-RFP IR(100 mW, 0.5 s) Olympus FV1000 PlanApo N 60 x NA1.42 oil Zoom x1
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Fig.6B HS-GFP his-RFP IR(100 mW, 0.5 s) Olympus FV1000 PlanApo N 60 x NA1.42 oil Zoom x1
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Fig.6C ERK FRET Ch1_DAPI_Ch2_FRETprobe_Ch3_dpERK Olympus FV1000 PlanApo N 60 x NA1.42 oil Zoom x2
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Fig.6D ERK FRET Sqh-mcherry IR(150 mW 0.5 s) Olympus FV1000 PlanApo N 60 x NA1.42 oil Zoom x3
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Fig.6E Control Par6-GFP his-RFP (150 mW 0.125 s) Olympus FV1000 PlanApo N 60 x NA1.42 oil Zoom x1
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Fig.6F egfr[f24] Par6-GFP his-RFP (150 mW 0.125 s) Olympus FV1000 PlanApo N 60 x NA1.42 oil Zoom x1
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