INFO:__main__:['data/20211022_cAMP_ERK/DRD1_2/DRD1_t001_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t002_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t003_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t004_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t005_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t006_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t007_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t008_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t009_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t010_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t011_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t012_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t013_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t014_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t015_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t016_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t017_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t018_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t019_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t020_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t021_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t022_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t023_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t024_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t025_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t026_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t027_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t028_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t029_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t030_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t031_c002.tif']
INFO:preprocess:Functions ['gaussian_blur'] for 31 images.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t001_c002.tif
INFO:preprocess:	frame 0 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t002_c002.tif
INFO:preprocess:	frame 1 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t003_c002.tif
INFO:preprocess:	frame 2 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t004_c002.tif
INFO:preprocess:	frame 3 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t005_c002.tif
INFO:preprocess:	frame 4 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t006_c002.tif
INFO:preprocess:	frame 5 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t007_c002.tif
INFO:preprocess:	frame 6 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t008_c002.tif
INFO:preprocess:	frame 7 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t009_c002.tif
INFO:preprocess:	frame 8 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t010_c002.tif
INFO:preprocess:	frame 9 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t011_c002.tif
INFO:preprocess:	frame 10 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t012_c002.tif
INFO:preprocess:	frame 11 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t013_c002.tif
INFO:preprocess:	frame 12 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t014_c002.tif
INFO:preprocess:	frame 13 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t015_c002.tif
INFO:preprocess:	frame 14 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t016_c002.tif
INFO:preprocess:	frame 15 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t017_c002.tif
INFO:preprocess:	frame 16 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t018_c002.tif
INFO:preprocess:	frame 17 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t019_c002.tif
INFO:preprocess:	frame 18 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t020_c002.tif
INFO:preprocess:	frame 19 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t021_c002.tif
INFO:preprocess:	frame 20 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t022_c002.tif
INFO:preprocess:	frame 21 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t023_c002.tif
INFO:preprocess:	frame 22 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t024_c002.tif
INFO:preprocess:	frame 23 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t025_c002.tif
INFO:preprocess:	frame 24 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t026_c002.tif
INFO:preprocess:	frame 25 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t027_c002.tif
INFO:preprocess:	frame 26 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t028_c002.tif
INFO:preprocess:	frame 27 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t029_c002.tif
INFO:preprocess:	frame 28 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t030_c002.tif
INFO:preprocess:	frame 29 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t031_c002.tif
INFO:preprocess:	frame 30 done.
INFO:__main__:['output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t001_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t002_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t003_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t004_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t005_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t006_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t007_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t008_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t009_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t010_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t011_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t012_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t013_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t014_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t015_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t016_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t017_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t018_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t019_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t020_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t021_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t022_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t023_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t024_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t025_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t026_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t027_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t028_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t029_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t030_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t031_c002.tif']
INFO:segment:Functions ['adaptive_thres'] for 31 images.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t001_c002.tif
INFO:segment:	frame 0: 72 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t002_c002.tif
INFO:segment:	frame 1: 71 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t003_c002.tif
INFO:segment:	frame 2: 71 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t004_c002.tif
INFO:segment:	frame 3: 70 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t005_c002.tif
INFO:segment:	frame 4: 70 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t006_c002.tif
INFO:segment:	frame 5: 70 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t007_c002.tif
INFO:segment:	frame 6: 70 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t008_c002.tif
INFO:segment:	frame 7: 70 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t009_c002.tif
INFO:segment:	frame 8: 70 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t010_c002.tif
INFO:segment:	frame 9: 70 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t011_c002.tif
INFO:segment:	frame 10: 71 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t012_c002.tif
INFO:segment:	frame 11: 70 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t013_c002.tif
INFO:segment:	frame 12: 70 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t014_c002.tif
INFO:segment:	frame 13: 71 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t015_c002.tif
INFO:segment:	frame 14: 71 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t016_c002.tif
INFO:segment:	frame 15: 70 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t017_c002.tif
INFO:segment:	frame 16: 71 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t018_c002.tif
INFO:segment:	frame 17: 70 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t019_c002.tif
INFO:segment:	frame 18: 70 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t020_c002.tif
INFO:segment:	frame 19: 70 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t021_c002.tif
INFO:segment:	frame 20: 70 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t022_c002.tif
INFO:segment:	frame 21: 70 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t023_c002.tif
INFO:segment:	frame 22: 69 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t024_c002.tif
INFO:segment:	frame 23: 69 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t025_c002.tif
INFO:segment:	frame 24: 69 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t026_c002.tif
INFO:segment:	frame 25: 69 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t027_c002.tif
INFO:segment:	frame 26: 69 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t028_c002.tif
INFO:segment:	frame 27: 69 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t029_c002.tif
INFO:segment:	frame 28: 69 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t030_c002.tif
INFO:segment:	frame 29: 70 objects segmented.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/at/DRD1_t031_c002.tif
INFO:segment:	frame 30: 70 objects segmented.
INFO:__main__:['output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t001_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t002_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t003_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t004_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t005_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t006_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t007_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t008_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t009_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t010_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t011_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t012_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t013_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t014_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t015_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t016_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t017_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t018_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t019_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t020_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t021_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t022_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t023_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t024_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t025_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t026_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t027_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t028_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t029_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t030_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t031_c002.tif']
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t001_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 16
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 3
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 2
INFO:track:	frame 0: 68 objects linked and 2 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t002_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 5
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 3
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 1
INFO:track:	frame 1: 69 objects linked and 1 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t003_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 10
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 3
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 0
INFO:track:	frame 2: 69 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t004_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 3
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 2
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 0
INFO:track:	frame 3: 69 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t005_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 69
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 1
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 0
INFO:track:	frame 4: 69 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t006_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 69
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 6
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 3
INFO:track:	frame 5: 66 objects linked and 3 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t007_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 67
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 1
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 1
INFO:track:	frame 6: 68 objects linked and 1 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t008_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 66
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 2
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 1
INFO:track:	frame 7: 69 objects linked and 1 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t009_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 58
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 2
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 0
INFO:track:	frame 8: 69 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t010_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 70
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 5
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 1
INFO:track:	frame 9: 69 objects linked and 1 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t011_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 27
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 1
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 0
INFO:track:	frame 10: 69 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t012_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 61
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 4
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 1
INFO:track:	frame 11: 68 objects linked and 1 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t013_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 16
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 3
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 1
INFO:track:	frame 12: 69 objects linked and 1 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t014_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 9
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 4
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 2
INFO:track:	frame 13: 68 objects linked and 2 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t015_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 11
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 1
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 0
INFO:track:	frame 14: 69 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t016_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 8
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 2
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 1
INFO:track:	frame 15: 69 objects linked and 1 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t017_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 32
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 1
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 0
INFO:track:	frame 16: 69 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t018_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 4
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 0
INFO:track:	frame 17: 69 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t019_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 11
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 2
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 0
INFO:track:	frame 18: 69 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t020_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 22
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 1
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 0
INFO:track:	frame 19: 69 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t021_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 2
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 1
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 0
INFO:track:	frame 20: 69 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t022_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 30
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 3
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 0
INFO:track:	frame 21: 68 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t023_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 23
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 1
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 0
INFO:track:	frame 22: 68 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t024_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 17
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 0
INFO:track:	frame 23: 68 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t025_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 3
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 0
INFO:track:	frame 24: 68 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t026_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 6
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 1
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 0
INFO:track:	frame 25: 68 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t027_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 3
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 0
INFO:track:	frame 26: 68 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t028_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 6
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 1
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 1
INFO:track:	frame 27: 67 objects linked and 1 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t029_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 3
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 2
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 1
INFO:track:	frame 28: 68 objects linked and 1 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t030_c002.tif
DEBUG:track:	run_lap with {'DISPLACEMENT': 10, 'MASSTHRES': 0.15}: 4
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 60, 'MASSTHRES': 0.15}: 3
DEBUG:track:	track_neck_cut with {'DISPLACEMENT': 10, 'MASSTHRES': 0.4}: 0
INFO:track:	frame 29: 69 objects linked and 0 unlinked.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/tracked/DRD1_t031_c002.tif
INFO:__main__:['output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t001_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t002_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t003_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t004_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t005_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t006_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t007_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t008_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t009_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t010_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t011_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t012_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t013_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t014_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t015_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t016_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t017_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t018_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t019_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t020_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t021_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t022_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t023_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t024_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t025_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t026_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t027_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t028_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t029_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t030_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t031_c002.tif']
INFO:postprocess:Postprocess.	collecting cells...
INFO:postprocess:	running cut_short_traces
INFO:postprocess:	saving images...
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t001_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t002_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t003_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t004_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t005_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t006_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t007_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t008_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t009_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t010_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t011_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t012_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t013_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t014_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t015_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t016_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t017_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t018_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t019_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t020_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t021_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t022_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t023_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t024_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t025_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t026_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t027_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t028_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t029_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t030_c002.tif
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/nuc/DRD1_t031_c002.tif
INFO:__main__:['output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t001_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t002_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t003_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t004_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t005_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t006_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t007_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t008_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t009_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t010_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t011_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t012_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t013_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t014_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t015_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t016_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t017_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t018_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t019_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t020_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t021_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t022_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t023_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t024_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t025_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t026_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t027_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t028_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t029_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t030_c002.tif', 'output/20211022_cAMP_ERK/DRD1_2/gb/DRD1_t031_c002.tif']
INFO:subdetect:Functions ['ring_dilation_above_offset_buffer'] for 31 images.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t001_c002.tif
INFO:subdetect:	frame 0 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t002_c002.tif
INFO:subdetect:	frame 1 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t003_c002.tif
INFO:subdetect:	frame 2 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t004_c002.tif
INFO:subdetect:	frame 3 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t005_c002.tif
INFO:subdetect:	frame 4 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t006_c002.tif
INFO:subdetect:	frame 5 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t007_c002.tif
INFO:subdetect:	frame 6 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t008_c002.tif
INFO:subdetect:	frame 7 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t009_c002.tif
INFO:subdetect:	frame 8 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t010_c002.tif
INFO:subdetect:	frame 9 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t011_c002.tif
INFO:subdetect:	frame 10 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t012_c002.tif
INFO:subdetect:	frame 11 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t013_c002.tif
INFO:subdetect:	frame 12 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t014_c002.tif
INFO:subdetect:	frame 13 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t015_c002.tif
INFO:subdetect:	frame 14 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t016_c002.tif
INFO:subdetect:	frame 15 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t017_c002.tif
INFO:subdetect:	frame 16 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t018_c002.tif
INFO:subdetect:	frame 17 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t019_c002.tif
INFO:subdetect:	frame 18 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t020_c002.tif
INFO:subdetect:	frame 19 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t021_c002.tif
INFO:subdetect:	frame 20 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t022_c002.tif
INFO:subdetect:	frame 21 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t023_c002.tif
INFO:subdetect:	frame 22 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t024_c002.tif
INFO:subdetect:	frame 23 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t025_c002.tif
INFO:subdetect:	frame 24 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t026_c002.tif
INFO:subdetect:	frame 25 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t027_c002.tif
INFO:subdetect:	frame 26 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t028_c002.tif
INFO:subdetect:	frame 27 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t029_c002.tif
INFO:subdetect:	frame 28 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t030_c002.tif
INFO:subdetect:	frame 29 done.
DEBUG:utils.file_io:Image (shape (512, 512)) is saved at output/20211022_cAMP_ERK/DRD1_2/cyto/DRD1_t031_c002.tif
INFO:subdetect:	frame 30 done.
INFO:__main__:[('data/20211022_cAMP_ERK/DRD1_2/DRD1_t001_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t001_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t001_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t001_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t002_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t002_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t002_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t002_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t003_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t003_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t003_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t003_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t004_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t004_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t004_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t004_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t005_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t005_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t005_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t005_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t006_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t006_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t006_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t006_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t007_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t007_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t007_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t007_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t008_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t008_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t008_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t008_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t009_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t009_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t009_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t009_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t010_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t010_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t010_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t010_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t011_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t011_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t011_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t011_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t012_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t012_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t012_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t012_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t013_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t013_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t013_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t013_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t014_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t014_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t014_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t014_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t015_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t015_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t015_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t015_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t016_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t016_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t016_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t016_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t017_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t017_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t017_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t017_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t018_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t018_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t018_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t018_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t019_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t019_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t019_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t019_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t020_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t020_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t020_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t020_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t021_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t021_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t021_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t021_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t022_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t022_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t022_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t022_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t023_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t023_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t023_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t023_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t024_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t024_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t024_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t024_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t025_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t025_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t025_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t025_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t026_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t026_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t026_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t026_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t027_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t027_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t027_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t027_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t028_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t028_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t028_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t028_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t029_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t029_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t029_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t029_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t030_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t030_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t030_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t030_c004.tif'), ('data/20211022_cAMP_ERK/DRD1_2/DRD1_t031_c001.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t031_c002.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t031_c003.tif', 'data/20211022_cAMP_ERK/DRD1_2/DRD1_t031_c004.tif')]
INFO:apply:Channel iRFP: nuc applied...
INFO:apply:	making dataframe...
INFO:apply:Channel iRFP: cyto applied...
INFO:apply:	making dataframe...
INFO:apply:Channel RFP: nuc applied...
INFO:apply:	making dataframe...
INFO:apply:Channel RFP: cyto applied...
INFO:apply:	making dataframe...
INFO:apply:Channel FRET: nuc applied...
INFO:apply:	making dataframe...
INFO:apply:Channel FRET: cyto applied...
INFO:apply:	making dataframe...
INFO:apply:Channel CFP: nuc applied...
INFO:apply:	making dataframe...
INFO:apply:Channel CFP: cyto applied...
INFO:apply:	making dataframe...
INFO:apply:	df.npz saved.
INFO:__main__:Caller finished.