Name : Photomanipulation318 01/09/2021 3:58:22 PM Type : 14 bit grey/pseudo Intensity : 1.000000 lux x : 512 * 0.666667 : um y : 512 * 0.666667 : um Wavelength : 2 Time : 20 [Environment] User=iQ Workstation Configuration=ALC CSUX STAGE FRAPPA [Environment End] [Created] Date=01/09/2021 Time=3:57:00 PM [Created End] [Protocol Description] Protocol - Photomanipulation Image - Photomanipulation Protocol Type - Time Series Camera Binning - Use Current Wait Allocation - On Camera Selection - Use Current Events Keyboard Events - On Default name - Spacebar Repeat T - 5 times (200 ms) Repeat - Channel (MP365_img561) Channel Description Channel - MP365_img561 Camera EM Gain - 999 Camera Binning X - CURRENT Camera Binning Y - CURRENT Camera Exposure Time (s) - 0.168067 Camera Exposure Units - 1 LUT - Grey Map Min - 468 Map Max - 15570 Trigger - Pre : X,X,X,X,X,X,X,X, :: Post : X,X,X,X,X,X,X,X, FRAPPA - Current Number Of Repeats - 1 Repetition Rate - 12 IX81_BX61 EPI Filter - 2 IX81_BX61 BF Shutter - Closed IX81_BX61 EPI Shutter - Closed IX81_BX61 ND Filter - 5 IX81_BX61 Filter 1 - 0 IX81_BX61 Filter 2 - 0 IX81_BX61 Filter 3 - 2 IX81_BX61 Port - 1 LVPZT - Current CSU Aperture - Current CSU Camera Port - Current CSU Lens 1 - Current CSU Lens 2 - Current CSU NIR Shutter - Current CSU PB Mirror - Current CSU PB Shutter - Current CSU Port - Current CSU Shutter - Open Dichroic - 1 Emmision - 3 Excitation - 1 NDFilter - Current AOTF Channel 1 Intensity - 0 AOTF Channel 2 Intensity - 0 AOTF Channel 3 Intensity - 95.2 AOTF Channel 4 Intensity - 35.5 AOTF Channel 5 Intensity - Current AOTF Channel 6 Intensity - Current AOTF Laser 1 Enabled - 1 AOTF Laser 1 External Control - Current AOTF Laser 2 Enabled - 1 AOTF Laser 2 External Control - Current AOTF Laser 3 Enabled - 1 AOTF Laser 3 External Control - Current AOTF Laser 4 Enabled - 1 AOTF Laser 4 External Control - Current AOTF Laser 5 Enabled - 0 AOTF Laser 5 External Control - Current AOTF Laser 6 Enabled - 0 AOTF Laser 6 External Control - Current AOTF MPD - 2 AOTF Position - 561 AOTF Shutter - Open Snap - Frame End - Channel Repeat - Channel (MP365_img488) Channel Description Channel - MP365_img488 Camera EM Gain - 600 Camera Binning X - CURRENT Camera Binning Y - CURRENT Camera Exposure Time (s) - 0.5 Camera Exposure Units - 1 LUT - Grey Map Min - 376 Map Max - 16029 Trigger - Pre : X,X,X,X,X,X,X,X, :: Post : X,X,X,X,X,X,X,X, FRAPPA - Current Number Of Repeats - 10 Repetition Rate - 16 IX81_BX61 EPI Filter - 2 IX81_BX61 BF Shutter - Closed IX81_BX61 EPI Shutter - Closed IX81_BX61 ND Filter - 5 IX81_BX61 Filter 1 - 0 IX81_BX61 Filter 2 - 0 IX81_BX61 Filter 3 - 2 IX81_BX61 Port - 1 LVPZT - Current CSU Aperture - Current CSU Camera Port - Current CSU Lens 1 - Current CSU Lens 2 - Current CSU NIR Shutter - Current CSU PB Mirror - Current CSU PB Shutter - Current CSU Port - Current CSU Shutter - Open Dichroic - 2 Emmision - 2 Excitation - 1 NDFilter - Current AOTF Channel 1 Intensity - 0 AOTF Channel 2 Intensity - 0 AOTF Channel 3 Intensity - 71 AOTF Channel 4 Intensity - 0 AOTF Channel 5 Intensity - Current AOTF Channel 6 Intensity - Current AOTF Laser 1 Enabled - 1 AOTF Laser 1 External Control - Current AOTF Laser 2 Enabled - 1 AOTF Laser 2 External Control - Current AOTF Laser 3 Enabled - 1 AOTF Laser 3 External Control - Current AOTF Laser 4 Enabled - 1 AOTF Laser 4 External Control - Current AOTF Laser 5 Enabled - 0 AOTF Laser 5 External Control - Current AOTF Laser 6 Enabled - 0 AOTF Laser 6 External Control - Current AOTF MPD - 2 AOTF Position - 488 AOTF Shutter - Open Snap - Frame End - Channel End - T Frappa - MP365 (MicroPoint, All, _FRAPPA) Camera EM Gain - CURRENT Camera Binning X - CURRENT Camera Binning Y - CURRENT Camera Exposure Time (s) - CURRENT Camera Exposure Units - CURRENT LUT - Grey Map Min - 83 Map Max - 350 Trigger - Pre : X,X,X,X,X,X,X,X, :: Post : X,X,X,X,X,X,X,X, FRAPPA - Current Number Of Repeats - 1 Repetition Rate - 12 IX81_BX61 EPI Filter - 2 IX81_BX61 BF Shutter - Current IX81_BX61 EPI Shutter - Current IX81_BX61 ND Filter - 5 IX81_BX61 Filter 1 - 0 IX81_BX61 Filter 2 - 0 IX81_BX61 Filter 3 - 2 IX81_BX61 Port - 1 LVPZT - Current CSU Aperture - Current CSU Camera Port - CURRENT CSU Lens 1 - Current CSU Lens 2 - Current CSU NIR Shutter - Current CSU PB Mirror - Current CSU PB Shutter - Current CSU Port - Current CSU Shutter - Current Dichroic - 1 Emmision - 2 Excitation - 1 NDFilter - Current AOTF Channel 1 Intensity - 0 AOTF Channel 2 Intensity - 0 AOTF Channel 3 Intensity - 0 AOTF Channel 4 Intensity - 0 AOTF Channel 5 Intensity - Current AOTF Channel 6 Intensity - Current AOTF Laser 1 Enabled - 1 AOTF Laser 1 External Control - Current AOTF Laser 2 Enabled - 1 AOTF Laser 2 External Control - Current AOTF Laser 3 Enabled - 1 AOTF Laser 3 External Control - Current AOTF Laser 4 Enabled - 1 AOTF Laser 4 External Control - Current AOTF Laser 5 Enabled - 0 AOTF Laser 5 External Control - Current AOTF Laser 6 Enabled - 0 AOTF Laser 6 External Control - Current AOTF MPD - 2 AOTF Position - 0 AOTF Shutter - Current Repeat T - 50 times (200 ms) Repeat - Channel (MP365_img561) Channel Description Channel - MP365_img561 Camera EM Gain - 999 Camera Binning X - CURRENT Camera Binning Y - CURRENT Camera Exposure Time (s) - 0.168067 Camera Exposure Units - 1 LUT - Grey Map Min - 468 Map Max - 15570 Trigger - Pre : X,X,X,X,X,X,X,X, :: Post : X,X,X,X,X,X,X,X, FRAPPA - Current Number Of Repeats - 1 Repetition Rate - 12 IX81_BX61 EPI Filter - 2 IX81_BX61 BF Shutter - Closed IX81_BX61 EPI Shutter - Closed IX81_BX61 ND Filter - 5 IX81_BX61 Filter 1 - 0 IX81_BX61 Filter 2 - 0 IX81_BX61 Filter 3 - 2 IX81_BX61 Port - 1 LVPZT - Current CSU Aperture - Current CSU Camera Port - Current CSU Lens 1 - Current CSU Lens 2 - Current CSU NIR Shutter - Current CSU PB Mirror - Current CSU PB Shutter - Current CSU Port - Current CSU Shutter - Open Dichroic - 1 Emmision - 3 Excitation - 1 NDFilter - Current AOTF Channel 1 Intensity - 0 AOTF Channel 2 Intensity - 0 AOTF Channel 3 Intensity - 95.2 AOTF Channel 4 Intensity - 35.5 AOTF Channel 5 Intensity - Current AOTF Channel 6 Intensity - Current AOTF Laser 1 Enabled - 1 AOTF Laser 1 External Control - Current AOTF Laser 2 Enabled - 1 AOTF Laser 2 External Control - Current AOTF Laser 3 Enabled - 1 AOTF Laser 3 External Control - Current AOTF Laser 4 Enabled - 1 AOTF Laser 4 External Control - Current AOTF Laser 5 Enabled - 0 AOTF Laser 5 External Control - Current AOTF Laser 6 Enabled - 0 AOTF Laser 6 External Control - Current AOTF MPD - 2 AOTF Position - 561 AOTF Shutter - Open Snap - Frame End - Channel Repeat - Channel (MP365_img488) Channel Description Channel - MP365_img488 Camera EM Gain - 600 Camera Binning X - CURRENT Camera Binning Y - CURRENT Camera Exposure Time (s) - 0.5 Camera Exposure Units - 1 LUT - Grey Map Min - 376 Map Max - 16029 Trigger - Pre : X,X,X,X,X,X,X,X, :: Post : X,X,X,X,X,X,X,X, FRAPPA - Current Number Of Repeats - 10 Repetition Rate - 16 IX81_BX61 EPI Filter - 2 IX81_BX61 BF Shutter - Closed IX81_BX61 EPI Shutter - Closed IX81_BX61 ND Filter - 5 IX81_BX61 Filter 1 - 0 IX81_BX61 Filter 2 - 0 IX81_BX61 Filter 3 - 2 IX81_BX61 Port - 1 LVPZT - Current CSU Aperture - Current CSU Camera Port - Current CSU Lens 1 - Current CSU Lens 2 - Current CSU NIR Shutter - Current CSU PB Mirror - Current CSU PB Shutter - Current CSU Port - Current CSU Shutter - Open Dichroic - 2 Emmision - 2 Excitation - 1 NDFilter - Current AOTF Channel 1 Intensity - 0 AOTF Channel 2 Intensity - 0 AOTF Channel 3 Intensity - 71 AOTF Channel 4 Intensity - 0 AOTF Channel 5 Intensity - Current AOTF Channel 6 Intensity - Current AOTF Laser 1 Enabled - 1 AOTF Laser 1 External Control - Current AOTF Laser 2 Enabled - 1 AOTF Laser 2 External Control - Current AOTF Laser 3 Enabled - 1 AOTF Laser 3 External Control - Current AOTF Laser 4 Enabled - 1 AOTF Laser 4 External Control - Current AOTF Laser 5 Enabled - 0 AOTF Laser 5 External Control - Current AOTF Laser 6 Enabled - 0 AOTF Laser 6 External Control - Current AOTF MPD - 2 AOTF Position - 488 AOTF Shutter - Open Snap - Frame End - Channel End - T [Protocol Description End] [XYZScan] ScanName= FileVersion=2 Centre=False Channel= WellPattern= Density=0 Calibration= XYFields= ZScan= Montage= Plates= MontageOffsets= MF Scan Present=False [XYZScan End] [Image Bits] 14 Bit Data=TRUE [Image Bits End] [Grab Parameters] Camera=Andor Window Left=0 Window Top=0 Window Right=511 Window Bottom=511 Window Width=512 Window Height=512 BinningX=1 BinningY=1 Image Width=512 Image Height=512 Exposure=168.067 ms Rotation=2 FlipV=0 FlipH=1 TimeStamps=Software [Grab Parameters End] [Tab Device Info] Camera=DU897_BV 6195 ControllerCard=CCI-23 DriverVersion=2.95.33002.0 CofVersion=291 VxDVersion=4.30 SoftwareTrigger=Supported BitDepth=14 FPGA_Version=7.9 Ocx_Version=5.4.8.64 Ocx_Time=11:04:32 Jun 04 2013 Ocx_File=C:\PROGRA~1\ANDORB~1\COMMON~1\KIDAND~1.OCX [Tab Device Info End] [Tab Exposure] Window=512 : 512 Shutter=Open Binning=1 x 1 Temperature - Actual =-75 Display Control - Fastest=Checked Display Control - Display Rate=1 Gains - Pre-Amp-Gain=5.1x EM Gain - Enabled=Checked Gains - EMGain=999 Exposure Time - Frame Transfer=Checked Exposure Time - unit=ms Exposure Time - exposure value=168.067 Exposure Time - Readout in ms=30.350 Experiment Control - FLZ=Unchecked Experiment Control - Trig Mode=Free Run Use Frame averaging=Unchecked Frames to be averaged=2 Fastest Grab=Unchecked Available memory=2047 [Tab Exposure End] [Tab Temperature/Shutter] Temperature Setting=-75 Actual Temperature=-75 Cooling=Steady Fan=Full TTL High Open=Checked Shutter Open Transfer Time=60 Shutter Close Transfer Time=80 [Tab Temperature/Shutter End] [Tab Speed/Amplifer] Horizontal Readout=10.000 Vertical Readout time=[1.700] ADC Channel=0 Vertical Clock Voltage=Normal Output Amplifier=EM Port Baseline Clamp=Checked [Tab Speed/Amplifer End] [Tab Image Orientation] Flip=Horizontal Rotation in Degrees=Anti-Clockwise 90 [Tab Image Orientation End] [Image Info] Window Left=0 Window Top=0 Window Right=511 Window Bottom=511 Window Width=512 Window Height=512 Window BinningX=1 Window BinningY=1 [Image Info End] [First Frame] SavedTime=9/1/2021 3:57:19 PM.671 [First Frame End] [Event Markers] Marker 1=Execute FRAPPA Node Camera EB Gain Camera Binning X Camera Binning Y Camera Exposure Time Camera Exposure Units LUT Map Min Map Max Trigger X Motor Y Motor Z Motor FRAPPA Number Of Repeats Repetition Rate IX81_BX61 Z IX81_BX61 EPI Filter IX81_BX61 BF Shutter IX81_BX61 EPI Shutter IX81_BX61 ND Filter IX81_BX61 Filter 1 IX81_BX61 Filter 2 IX81_BX61 Filter 3 IX81_BX61 Port LVPZT CSU Aperture CSU Camera Port CSU Lens 1 CSU Lens 2 CSU NIR Shutter CSU PB Mirror CSU PB Shutter CSU Port CSU Shutter Dichroic Emmision Excitation NDFilter AOTF Channel 1 Intensity AOTF Channel 2 Intensity AOTF Channel 3 Intensity AOTF Channel 4 Intensity AOTF Channel 5 Intensity AOTF Channel 6 Intensity AOTF Laser 1 Enabled AOTF Laser 1 External Control AOTF Laser 2 Enabled AOTF Laser 2 External Control AOTF Laser 3 Enabled AOTF Laser 3 External Control AOTF Laser 4 Enabled AOTF Laser 4 External Control AOTF Laser 5 Enabled AOTF Laser 5 External Control AOTF Laser 6 Enabled AOTF Laser 6 External Control AOTF MPD AOTF Position AOTF Shutter MP365 CURRENT CURRENT CURRENT CURRENT CURRENT Grey 83 350 Pre frame Trigger : Current,Current,Current,Current,Current,Current,Current,Current, :: Post frame Trigger : Current,Current,Current,Current,Current,Current,Current,Current, Current Current Current Current 1 12 Current 2 Current Current 5 0 0 2 1 Current Current CURRENT Current Current Current Current Current Current Current 1 2 1 Current 0 0 0 0 Current Current 1 Current 1 Current 1 Current 1 Current 0 Current 0 Current 2 0 Current MicroPoint _FRAPPA Line NumberOfPoints( 2) : ( 206, 324) ( 201, 334) Label( 1) Location 1=Wavelength(0(0)) Time(5(16894)) [Event Markers End]