This directory contains two image files for Figure 3.
Information on the sample, imaging condition, and files are described below.
Please also see Preparation and imaging for MDCK cells in the Method section

(1) Sample
Cells: Madin-Darby Canine Kidney (MDCK) cells cultured until reaching pre- and post-confluence
Fluorescent dye: NucleoSeeing, nucleus-staining green fluorescent chemical probe (Funakoshi, Tokyo, Japan)
Treatment: fixed with 4% paraformaldehyde (10 min), washed with PBS, permeabilized with 0.2% Triton X-100 (10 min)

(2) Image acquisition condition
Excitation wavelengths: 470 nm
Exposure time: 1 s

(3) Image files
File format: TIFF (16 Bit)
Effective number of pixels: 13,264 × 9180
Scaling: 1.1 um/pixel

“Fig3_MDCKpreconfl.tif” - 
nucleus image of MDCK cells in the pre-confluent state (used for Fig. 3a,b,d)
“Fig3_MDCKpostconfl.tif” - 
nucleus image of MDCK cells in the post-confluent state (used for Fig. 3c,e)

(4) Pre-processing applied to the image data
Both the image data were pre-processed from raw data by the following method.
a. Compensation of the non-uniformity of the excitation intensity and lens-throughput (Please see Presence and compensation of non-uniformity of light illumination and lens throughput in the Method section.)