Each file in this folder shows contour tracing of all the DG neurons that were manually identified in an individual animal.

The cells in the images are indicated with three different colors:
 -red and blue cells: activities were identified at least once in a 30-min open field session
 -black cells: no activity was identified during the session
 -red cross marks: misrecognized cells due to artifacts, which were excluded from the counting

Procedures used to create these images:
First, to visualize all the neurons clearly, projection of motion-corrected fluorescent image (MC*) and of its fluorescent change image (DF/F**), available in inscopix data processing software (IDPS), were generated. By placing a transparent projector sheet on the cell images in MC and DFF projections, the contours of individual neurons were manually traced with color markers. Afterward, these sheets were used to draw manual ROIs in IDPS, and calcium signal traces of all the individual neurons were generated. By manually examining calcium traces of each neuron, the presence of neuronal activity was examined one by one.

Remarks:
 *In the MC projection images, you can see all the neurons regardless of how active they are. Even the ones without any activity are visible in this image. (available in *.isxp files located at  /Decoding dataset_rawdata)
 **In the DF/F projection images, only the neurons that showed activitie(s) at least once during the recording are visible. (available as grayscale images in /Active cell count/Contour maps)


The analyses above were performed by:
Ryuichi Nakajima Ph.D., Fujita Health University

Special thanks to Yoshihiro Takamiya for technical assistance.