Each image file in this folder shows all the identifiable DG neurons (colored contours) overlaid on the images of the cells which showed activities (grayscale).

Procedures used to create these images:
First, to visualize all the neurons clearly, projection of motion-corrected fluorescent image (MC*) and of its fluorescent change image (DF/F**) were generated. By placing a transparent projector sheet on the cell images in MC and DFF projections, the contours of individual neurons were manually traced with color markers (/Manual ROI drawings_WT_HKO/). Afterward, these sheets were used to draw manual ROIs in IDPS, and calcium signal traces of all the individual neurons were generated. By manually examining calcium traces of each neuron, the presence of neuronal activity was examined one by one.

Remarks:
 *In the MC projection images, you can see all the neurons regardless of how active they are. Even the ones without any activity are visible in this image. (available in *.isxp files located at  /Decoding dataset_rawdata)
 **In the DF/F projection images, only the neurons that showed activities at least once during the recording are visible. (available as grayscale images in /Active cell count/Contour maps)


The analyses described above were performed by:
Ryuichi Nakajima Ph.D., Fujita Health University

Special thanks to Yoshihiro Takamiya for technical assistance.