SSBD:database

Project

Dataset ID

Fig6j_voltageOBdendrite_SDL

Kind

Image data

Title

Time-lapse images of glomerular layer in the olfactory bulb in vivo expressing Voltron2 after clearing with SeeDB-Live

Description

Time-lapse images of mitral cells expressing Voltron2 in the olfactory bulb of an anesthetized mouse after clearing with SeeDB-Live. Voltron2 was specifically and sparsely expressed in mitral/tufted cells using the Pcdh21-Cre driver and a Cre-dependent adeno-associated virus (AAV) vector, labeled with JF549-HaloTag ligand (Voltron2_{549}). Voltage change in the dendrites of the mitral cell in response to odor stimulation (1% amyl acetate) were imaged. The odor was delivered to a mouse nose for 5 seconds at 1 litter per minute.

Folder Name

Fig6j_voltageOBdendrite_SDL

File Format

.tif

File Size

750MB

File Separation Method

-

Figure Number

Fig6j-p

Dimensions

256x256x1x1x5714

X Scale

2.89 micrometer

Y Scale

2.89 micrometer

Z Scale

-

T Scale

7 milliseconds

Channel

1 channel

Quantitative Data

-

Description

An anesthetized Voltron2-expressing transgenic mouse (4-month-old). Craniotomy and durotomy were made on the right hemisphere. The brain surface was perfused with SeeDB-Live and perfused for 1 h under anesthesia.

Organism

Strain

-

Cell

neuron mitral cell (CL:1001502)

Cell line

-

MeSH

Intrinsic Variable

Pcdh21-Cre transgennic mouse was used.

Extrinsic Variable

To express Volton2 by Cre dependent manner, AAV-syn-FLEX-Volton2 was injected into the olfactory bulb of Pcdh21-Cre mouse. 50 nM Janelia Fluor HaloTag Ligand 549 was incubated with the slice before imaging.

Anatomical Entity

-

UBERON

Biological Process

Cellular Component

Molecular Function

-

MeSH Anatomy

MeSH BP

MeSH CC

MeSH MF

Imaging Method

Detection Method

high-speed CMOS camera

Visualization Method

Voltron2 was visualized with JF549-HaloTag ligand applied to the medium (Voltron2_{549})

Illumination Method

-

Source of Contrast

-

Imaged Parameter

fluorescence emission

Sample Preparation Method

living tissue tissue clearing

MeSH

Body

Olympus-Evident FV1000MPE

Module

-

Light Source

-

Detector

-

Objective

Olympus-Evident XLPLN25XWMP

Filter Set

-

Dichroic

-