484-Inagaki-SeeDBLive/Fig5no_L2and3_day80
Author reviewedTime-lapse calcium imaging of layer 2 and 3 neurons in primary somatosensory
cortex at Day80
Project
Dataset ID
Fig5no_L2and3_day80
Kind
Image data
Title
Time-lapse calcium imaging of layer 2 and 3 neurons in primary somatosensory
cortex at Day80
Description
Long-term monitoring of neuronal activity in awake mice. Imaging of one mouse was conducted four trials at Day7, Day80, Day100, and Day120. This dataset includes the data of Day80. Layer 2/3 neurons in somatosensory cortex were labeled with AAV-jGCaMP8m-P2A-CyRFP1. Activity of neurons in response to whisker stimulations (five times) with air puffs were detected. The brain surface was perfused with SeeDB-Live during imaging. 2 channels are stacked into a single file. 1-1610 frames for jGCaMP8m, 1612-3221 frames for CyRFP1.
Folder Name
Fig5no_L2and3_day80
File Format
.tif
File Size
1.6GB
File Separation Method
2 channels are stacked into a single file. 1-1610 frames for jGCaMP8m, 1612-3221 frames for CyRFP1
Figure Number
Fig5no
Dimensions
512x512x7x1x230
X Scale
1.043 micrometer
Y Scale
1.043 micrometer
Z Scale
20 micrometer
T Scale
0.405 seconds
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
An awake mouse injected AAV-jGCaMP8m-P2A-CyRFP1. jGCaMP8m and CyRFP1 was expressed in layer 2/3 neurons in primary somatosensory
cortex. Craniotomy and durotomy were made on the right cortex. The brain surface was perfused with SeeDB-Live.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron
cerebral cortex pyramidal neuron (CL:4023111)
Cell line
-
MeSH
Intrinsic Variable
-
Extrinsic Variable
L2/3 neurons in S1 were labeled with
AAV-jGCaMP8m-P2A-CyRFP1.
Ontology
Anatomical Entity
-
UBERON
brain (UBERON:0000955)
cerebral cortex (UBERON:0000956)
Biological Process
calcium ion transport (GO:0006816)
Cellular Component
cell body (GO:0044297)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
AAV-vector injection
Gene
-
Protein / Probe
-
Method / Genotype
AAV-vector injection
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
jGCaMP8m
CyRFP1
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
tissue clearing
MeSH
Instrument
Body
Thorlabs MM201
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-