SSBD:database

484-Inagaki-SeeDBLive/Fig5no_L2and3_day100

Author reviewed

Time-lapse calcium imaging of layer 2 and 3 neurons in primary somatosensory cortex at Day100

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Project

484-Inagaki-SeeDBLive

Dataset ID

Fig5no_L2and3_day100

Kind

Image data

Title

Time-lapse calcium imaging of layer 2 and 3 neurons in primary somatosensory cortex at Day100

Description

Long-term monitoring of neuronal activity in awake mice. Imaging of one mouse was conducted four trials at Day7, Day80, Day100, and Day120. This dataset includes the data of Day100. Layer 2/3 neurons in somatosensory cortex were labeled with AAV-jGCaMP8m-P2A-CyRFP1. Activity of neurons in response to whisker stimulations (five times) with air puffs were detected. The brain surface was perfused with SeeDB-Live during imaging.

Folder Name

Fig5no_L2and3_day100

File Format

.tif

File Size

1.7GB

File Separation Method

2 channels are stacked into a single file. 1-1750 frames for jGCaMP8m, 1752-3501 frames for CyRFP1

Figure Number

Fig5no

Dimensions

512x512x7x2x250

X Scale

1.043 micrometer

Y Scale

1.043 micrometer

Z Scale

20 micrometer

T Scale

0.405 seconds

Channel

2 channel

Quantitative Data

Paper(s)

Biosample

Description

An awake mouse injected AAV-jGCaMP8m-P2A-CyRFP1. jGCaMP8m and CyRFP1 was expressed in layer 2/3 neurons in primary somatosensory cortex. Craniotomy and durotomy were made on the right cortex. The brain surface was perfused with SeeDB-Live.

Organism

Mus musculus (NCBITaxon:10090)

Strain

Cell

neuron cerebral cortex pyramidal neuron (CL:4023111)

Cell line

MeSH

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Brain (D001921)

Calcium Signaling (D020013)

Cell Body (D066146)

Pyramidal Cells (D017966)

Time-Lapse Imaging (D059008)

Cerebral Cortex (D002540)

Intrinsic Variable

Extrinsic Variable

L2/3 neurons in S1 were labeled with AAV-jGCaMP8m-P2A-CyRFP1.

Ontology

Anatomical Entity

UBERON

brain (UBERON:0000955)

cerebral cortex (UBERON:0000956)

Biological Process

calcium ion transport (GO:0006816)

Cellular Component

cell body (GO:0044297)

Molecular Function

MeSH Anatomy

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Brain (D001921)

Calcium Signaling (D020013)

Cell Body (D066146)

Pyramidal Cells (D017966)

Time-Lapse Imaging (D059008)

Cerebral Cortex (D002540)

MeSH BP

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Brain (D001921)

Calcium Signaling (D020013)

Cell Body (D066146)

Pyramidal Cells (D017966)

Time-Lapse Imaging (D059008)

Cerebral Cortex (D002540)

MeSH CC

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Brain (D001921)

Calcium Signaling (D020013)

Cell Body (D066146)

Pyramidal Cells (D017966)

Time-Lapse Imaging (D059008)

Cerebral Cortex (D002540)

MeSH MF

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Brain (D001921)

Calcium Signaling (D020013)

Cell Body (D066146)

Pyramidal Cells (D017966)

Time-Lapse Imaging (D059008)

Cerebral Cortex (D002540)

Genetics

Gene

Protein / Probe

Method / Genotype

AAV-vector injection

Gene

Protein / Probe

Method / Genotype

AAV-vector injection

Treatments

Reagent / Compound

Concentration

Fold Dilution

Imaging Method

two-photon laser scanning microscopy (FBbi:00000254)

time lapse microscopy (FBbi:00000249)

Detection Method

Visualization Method

jGCaMP8m CyRFP1

Illumination Method

Source of Contrast

Imaged Parameter

fluorescence emission

Sample Preparation Method

living tissue tissue clearing

MeSH

Neurons (D009474)

Microscopy, Fluorescence, Multiphoton (D036641)

Brain (D001921)

Calcium Signaling (D020013)

Cell Body (D066146)

Pyramidal Cells (D017966)

Time-Lapse Imaging (D059008)

Cerebral Cortex (D002540)

Instrument

Body

Thorlabs MM201

Module

Light Source

Detector

Objective

Olympus-Evident XLPLN25XWMP

Filter Set

Dichroic