484-Inagaki-SeeDBLive/Fig3o_P13_SDL
Author reviewedTime-lapse calcium imaging of mouse olfactory bulb slice in SeeDB-Live
Project
Dataset ID
Fig3o_P13_SDL
Kind
Image data
Title
Time-lapse calcium imaging of mouse olfactory bulb slice in SeeDB-Live
Description
Time-lapse calcium imaging of mouse olfactory bulb slice in SeeDB-Live. GCaMP6f was expressed in the mitral cells of the olfactory bulb, and it was imaged by confocal microscopy. Spontaneous activities of the neurons at a depth of 100 micrometer from the surface of the slice were imaged.
Folder Name
Fig3o_P13_SDL
File Format
.tif
File Size
16MB
File Separation Method
-
Figure Number
Fig3o
Dimensions
382x363x1x1x60
X Scale
0.994 micrometer
Y Scale
0.994 micrometer
Z Scale
-
T Scale
1.109 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)
Biosample
Description
An acute olfactory bulb slice from mouse at postnatal day 13, which was immersed in SeeDB-Live. GCaMP6f was expressed in mitral cells of the olfactory bulb.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
A mouse line Thy1-GCaMP6f in which GCaMP6f is expressed in neurons.
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
Cellular Component
cell body (GO:0044297)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
GCaMP6f
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
tissue clearing
MeSH
Instrument
Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-