SSBD:database

484-Inagaki-SeeDBLive/Fig3l_150um_SDL thumbnail

484-Inagaki-SeeDBLive/Fig3l_150um_SDL

Author reviewed
Time-lapse calcium imaging of mouse olfactory bulb slice during immersing in SeeDB-Live
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SSBD:downloads
Project
484-Inagaki-SeeDBLive
Dataset ID
Fig3l_150um_SDL
Kind
Image data
Title
Time-lapse calcium imaging of mouse olfactory bulb slice during immersing in SeeDB-Live
Description
Time-lapse calcium imaging of mouse olfactory bulb slice during immersing in SeeDB-Live. In the mitral cells of the olfactory bulb, GCaMP6f was expressed. Spontaneous activities of the neurons at a depth of 150 micrometer from the surface of the slice were imaged during clearing with SeeDB-Live. The culture medium is replaced from ACSF to SeeDB-Live over time.
Folder Name
Fig3l_150um_SDL_1
File Format
.tif
File Size
4.5GB
File Separation Method
-
Figure Number
Fig3l
Dimensions
662x662x1x1x5525
X Scale
0.72 micrometer
Y Scale
0.72 micrometer
Z Scale
-
T Scale
1.110 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
An acute olfactory bulb slice from mouse at postnatal day 9, which was immersed in SeeDB-Live. GCaMP6f was expressed in mitral cells of the olfactory bulb.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
Intrinsic Variable
A mouse line Thy1-GCaMP6f in which GCaMP6f is expressed in neurons.
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
Cellular Component
cell body (GO:0044297)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
GCaMP6f
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue tissue clearing
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-