SSBD:database

484-Inagaki-SeeDBLive/Fig3jk_NMDAstim_SDL_3 thumbnail

484-Inagaki-SeeDBLive/Fig3jk_NMDAstim_SDL_3

Author reviewed
Time-lapse calcium imaging of mouse olfactory bulb stimulated with NMDA
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Project
484-Inagaki-SeeDBLive
Dataset ID
Fig3jk_NMDAstim_SDL_3
Kind
Image data
Title
Time-lapse calcium imaging of mouse olfactory bulb stimulated with NMDA
Description
Time-lapse calcium imaging of mouse olfactory bulb slice immersed in SeeDB-Live. In the mitral cells of the olfactory bulb, GCaMP6f was expressed. Activities of the neurons stimulated with NMDA and glycine at a depth of 100 micrometer from the surface of the slice were imaged. This dataset includes the images before, during and after washing out the stimulant.
Folder Name
Fig3jk_NMDAstim_SDL_3
File Format
.tif
File Size
213MB
File Separation Method
-
Figure Number
Fig3jk
Dimensions
512x512x1x1x406
X Scale
0.994 micrometer
Y Scale
0.994 micrometer
Z Scale
-
T Scale
1.109 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
An acute olfactory bulb slice from mouse at postnatal day 15, which was immersed in SeeDB-Live. GCaMP6f was expressed in mitral cells of the olfactory bulb.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
Intrinsic Variable
A mouse line Thy1-GCaMP6f in which GCaMP6f is expressed in neurons.
Extrinsic Variable
The olfactory bulb slice was treated with NMDA and glycine during imaging

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
Cellular Component
cell body (GO:0044297)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J

Treatments

Reagent / Compound
N-methyl-D-aspartic acid
Concentration
100 micromolar
Fold Dilution
-
Reagent / Compound
glycine
Concentration
40 micromolar
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
GCaMP6f
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue tissue clearing
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-