SSBD:database

484-Inagaki-SeeDBLive/ExtFig9hi_InVivoVoltage_awake_mTurquoise thumbnail

484-Inagaki-SeeDBLive/ExtFig9hi_InVivoVoltage_awake_mTurquoise

Author reviewed
Z-series images of mTurquoise2 labelled L2/3 neurons in S1 of awake mouse
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Project
484-Inagaki-SeeDBLive
Dataset ID
ExtFig9hi_InVivoVoltage_awake_mTurquoise
Kind
Image data
Title
Z-series images of mTurquoise2 labelled L2/3 neurons in S1 of awake mouse
Description
Z-series images of mTurquoise2-expressing layer 2/3 neurons of somatosensory cortex in awake mouse. mTurquoise2 and Voltron2-ST were sparsely introduced into L2/3 neurons by in utero electroporation. The brain surface was perfused with SeeDB-Live during imaging. This dataset includes the images of mTurquoise2.
Folder Name
ExtFig9hi_InVivoVoltage_awake_mTurquoise
File Format
.tif
File Size
40MB
File Separation Method
-
Figure Number
Extended Data Fig9
Dimensions
512x512x77x1x1
X Scale
0.994 micrometer
Y Scale
0.994 micrometer
Z Scale
3 micrometer
T Scale
-
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
An awake mouse expressing Voltron2-ST and mTurquoise2. Craniotomy and durotomy were made on the right hemisphere. The brain surface was perfused with SeeDB-Live.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron (CL:0000540)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Dendrites (D003712)
Cerebral Cortex (D002540)
Intrinsic Variable
-
Extrinsic Variable
pCAG-tTA2, pBS-TRE-mTQ2 and pGP-CAG-Voltron2 plasmids were introduced into the E15 brain by in utero electroporation. 50 nM Janelia Fluor HaloTag Ligand 549 was incubated before imaging.

Ontology

Anatomical Entity
-
UBERON
brain (UBERON:0000955)
cerebral cortex (UBERON:0000956)
Biological Process
-
Cellular Component
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Dendrites (D003712)
Cerebral Cortex (D002540)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Dendrites (D003712)
Cerebral Cortex (D002540)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Dendrites (D003712)
Cerebral Cortex (D002540)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Dendrites (D003712)
Cerebral Cortex (D002540)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transfection electroporation

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
mTurquoise2
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue tissue clearing
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Dendrites (D003712)
Cerebral Cortex (D002540)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-