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484-Inagaki-SeeDBLive/ExtFig9def_InVivoVoltage_anesth_SDL thumbnail

484-Inagaki-SeeDBLive/ExtFig9def_InVivoVoltage_anesth_SDL

Author reviewed
Time-lapse images of Voltron2-ST labelled L2/3 neurons in S1 of anesthetized mice after clearing with SeeDB-Live
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Project
484-Inagaki-SeeDBLive
Dataset ID
ExtFig9def_InVivoVoltage_anesth_SDL
Kind
Image data
Title
Time-lapse images of Voltron2-ST labelled L2/3 neurons in S1 of anesthetized mice after clearing with SeeDB-Live
Description
Time-lapse images of Voltron2-ST labelled neurons in layer 2/3 of somatosensory cortex of anesthetized mouse before clearing with SeeDB-Live. Voltron2-ST, a fast and sensitive chemigenetic voltage indicator was introduced to layer 2/3 neurons by in utero electroporation. Cell body of the neurons at 120-150 micrometer depth of the brain was imaged by epifluorescence microscopy. The brain surface was perfused with SeeDB-Live during imaging.
Folder Name
ExtFig9def_InVivoVoltage_anesth_SDL
File Format
.tif
File Size
13.1GB
File Separation Method
-
Figure Number
Extended Data Fig9
Dimensions
256x256x1x1x10000
X Scale
2.89 micrometer
Y Scale
2.89 micrometer
Z Scale
-
T Scale
1 milliseconds
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
An anesthetized mouse expressing Voltron2-ST. Craniotomy and durotomy were made on the right hemisphere. The brain surface was perfused with SeeDB-Live.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron (CL:0000540)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Brain (D001921)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)
Intrinsic Variable
-
Extrinsic Variable
Voltron2-ST was expressed in L2/3 neurons of S1

Ontology

Anatomical Entity
-
UBERON
brain (UBERON:0000955)
cerebral cortex (UBERON:0000956)
Biological Process
neuronal action potential propagation (GO:0019227)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Brain (D001921)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Brain (D001921)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Brain (D001921)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Brain (D001921)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transfection electroporation

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
fluorescence microscopy (FBbi:00000246)
time lapse microscopy (FBbi:00000249)
epifluorescence microscopy
Detection Method
high-speed CMOS camera
Visualization Method
Voltron2-ST was visualized with JF549–HaloTag ligand applied to the medium
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue tissue clearing
MeSH
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Brain (D001921)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-