SSBD:database

484-Inagaki-SeeDBLive/ExtFig8hi_2P_Voltage_L5 thumbnail

484-Inagaki-SeeDBLive/ExtFig8hi_2P_Voltage_L5

Author reviewed
Two-photon voltage imaging of JEDI-2P-labelled L5 neurons in S1 of awake mice
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Project
484-Inagaki-SeeDBLive
Dataset ID
ExtFig8hi_2P_Voltage_L5
Kind
Image data
Title
Two-photon voltage imaging of JEDI-2P-labelled L5 neurons in S1 of awake mice
Description
Two-photon voltage imaging of JEDI-2P-labelled neurons in layer 5 of somatosensory cortex of awake mice. The brain surface was perfused with SeeDB-Live, and neurons at 564 micrometer depth of the brain was imaged by two-photon confocal microscopy.
Folder Name
ExtFig8hi_2P_Voltage_L5
File Format
.tif
File Size
165MB
File Separation Method
-
Figure Number
Extended Data Fig8
Dimensions
128x128x1x1x5001
X Scale
0.912 micrometer
Y Scale
0.912 micrometer
Z Scale
-
T Scale
8.8 milliseconds
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
An awake mouse expressing JEDI-2P. Craniotomy and durotomy were made on the right hemisphere. The brain surface was perfused with SeeDB-Live and perfused for 1 h under anesthesia
Organism
Mus musculus (NCBITaxon:10090)
Strain
C57BL/6N (https://www.jax.org/strain/005304)
Cell
neuron extratelencephalic-projecting glutamatergic cortical neuron (CL:4023009)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Cell Body (D066146)
Pyramidal Cells (D017966)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)
Intrinsic Variable
-
Extrinsic Variable
AAV1:pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE and AAV1:pENN.AAV.CamKII 0.4.Cre.SV40 were injected at a 3:1 ratio into 2-month-old mouse.

Ontology

Anatomical Entity
-
UBERON
brain (UBERON:0000955)
cerebral cortex (UBERON:0000956)
Biological Process
-
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Cell Body (D066146)
Pyramidal Cells (D017966)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Cell Body (D066146)
Pyramidal Cells (D017966)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Cell Body (D066146)
Pyramidal Cells (D017966)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Cell Body (D066146)
Pyramidal Cells (D017966)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
AAV-vector injection

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
JEDI-2P
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue tissue clearing
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Cell Body (D066146)
Pyramidal Cells (D017966)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)

Instrument

Body
Thorlabs MM201
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-