484-Inagaki-SeeDBLive/ExtFig10_InVivoVoltage_glomeruli
Author reviewedTime-lapse voltage imaging of glomerular layer of the olfactory bulb in vivo using SeeDB-Live
Project
Dataset ID
ExtFig10_InVivoVoltage_glomeruli
Kind
Image data
Title
Time-lapse voltage imaging of glomerular layer of the olfactory bulb in vivo using SeeDB-Live
Description
Odor responses of mitral cells of the olfactory
bulb in anesthetized mouse. In the mitral cells, Voltron2 was expressed. Voltage change in the dendrites of the mitral cell in response to odor stimulation (1% amyl acetate) were imaged. The odor was delivered to a mouse nose for 5 seconds at 1 litter per minute. The brain surface was perfused with SeeDB-Live, and imaging was conducted before, during and after application of the odor.
Folder Name
ExtFig10_InVivoVoltage_glomeruli
File Format
.tif
File Size
750MB
File Separation Method
-
Figure Number
Extended Data Fig10
Dimensions
256x256x1x1x5714
X Scale
2.89 micrometer
Y Scale
2.89 micrometer
Z Scale
-
T Scale
7 milliseconds
Channel
1 channel
Quantitative Data
-
Paper(s)
Biosample
Description
An awake mouse expressing Voltron2. Craniotomy and durotomy were made on the right hemisphere. The brain surface was perfused with SeeDB-Live.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
Pcdh21-Cre transgennic mouse was used.
Extrinsic Variable
To express Volton2 by Cre dependent manner, AAV-syn-FLEX-Volton2 was injected into the olfactory bulb of Pcdh21-Cre mouse. 50 nM Janelia Fluor HaloTag Ligand 549 was incubated with the slice before imaging.
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
response to odorant (GO:1990834)
neuronal action potential propagation (GO:0019227)
neuronal action potential back-propagation (GO:0098873)
Cellular Component
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transgenic
AAV-vector injection / Pcdh21-Cre
Treatments
Reagent / Compound
pentyl acetate
Concentration
1 percent
Fold Dilution
-
Imaging Method
Imaging Method
fluorescence microscopy (FBbi:00000246)
epifluorescence microscopy
Detection Method
high-speed CMOS
camera
Visualization Method
Voltron2 was visualized with JF549–HaloTag
ligand applied to the medium (Voltron2_{549})
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
tissue clearing
MeSH
Instrument
Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-