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476-Matsuda-GreenRedFRET/CaImage_TwichGmRR thumbnail

476-Matsuda-GreenRedFRET/CaImage_TwichGmRR

Author reviewed
Calcium imaging of HeLa cells in response to histamine conducted with calcium indicator, Twitch-GmRR
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Project
476-Matsuda-GreenRedFRET
Dataset ID
CaImage_TwichGmRR
Kind
Image data
Title
Calcium imaging of HeLa cells in response to histamine conducted with calcium indicator, Twitch-GmRR
Description
Calcium imaging of HeLa cells in response to histamine, which was conducted with calcium indicator, Twitch-GmRR. Twitch-GmRR Is an improved FRET-based calcium indicator with green and red fluorescent protein pairs.
Folder Name
CaImage_TwichGmRR
File Format
.tif
File Size
320MB
File Separation Method
green and red channels are separated
Figure Number
Fig3AB
Dimensions
512x512x1x2x301
X Scale
1.875 micrometer
Y Scale
1.875 micrometer
Z Scale
-
T Scale
3 seconds
Channel
2 channel
Quantitative Data
-
Paper(s)

Biosample

Description
HeLa cells expressing calcium indicator, Twitch-GmRR.
Organism
Homo sapiens (NCBITaxon:9606)
Strain
-
Cell
-
Cell line
HeLa Cell (CLO:0003684)
MeSH
HeLa Cells (D006367)
Microscopy, Confocal (D018613)
Cells (D002477)
Histamine (D006632)
Cytosol (D003600)
Calcium Signaling (D020013)
Intrinsic Variable
-
Extrinsic Variable
Calcium indicator, Twitch-GmRR was transfected into the cells

Ontology

Anatomical Entity
-
UBERON
-
Biological Process
response to histamine (GO:0034776)
calcium ion import into cytosol (GO:1902656)
Cellular Component
cytosol (GO:0005829)
Molecular Function
-
MeSH Anatomy
HeLa Cells (D006367)
Microscopy, Confocal (D018613)
Cells (D002477)
Histamine (D006632)
Cytosol (D003600)
Calcium Signaling (D020013)
MeSH BP
HeLa Cells (D006367)
Microscopy, Confocal (D018613)
Cells (D002477)
Histamine (D006632)
Cytosol (D003600)
Calcium Signaling (D020013)
MeSH CC
HeLa Cells (D006367)
Microscopy, Confocal (D018613)
Cells (D002477)
Histamine (D006632)
Cytosol (D003600)
Calcium Signaling (D020013)
MeSH MF
HeLa Cells (D006367)
Microscopy, Confocal (D018613)
Cells (D002477)
Histamine (D006632)
Cytosol (D003600)
Calcium Signaling (D020013)

Genetics

Gene
-
Protein / Probe
-Twitch-GmRR
Method / Genotype
transfection

Treatments

Reagent / Compound
histamine
Concentration
0.5 millimolar
Fold Dilution
100 folds

Imaging Method

Imaging Method
spinning disk confocal microscopy (FBbi:00000253)
Detection Method
-
Visualization Method
Twitch-GmRR
Illumination Method
spinning disk scanning
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
HeLa Cells (D006367)
Microscopy, Confocal (D018613)
Cells (D002477)
Histamine (D006632)
Cytosol (D003600)
Calcium Signaling (D020013)

Instrument

Body
Nikon Eclipse Ti-E
Module
CREST Optics X-Light V2 (60 μm pinhole spinning disk)
Light Source
Lumencor CERESTA Light Engine
Detector
Andor iXon3 EM CCD
Objective
Nicon 60× oil immersion objective lens (NA = 1.40, Plan Apo)
Filter Set
emission filters 520/35 and 641/75 (Semrock)
Dichroic
-