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474-Yoshinari-NucleiAutofluoNIR/MovS4_NIR_Roquette_RootTip_nuclei thumbnail

474-Yoshinari-NucleiAutofluoNIR/MovS4_NIR_Roquette_RootTip_nuclei

Author reviewed
The time-lapse NIR autofluorescence imaging of nuclear dynamics in Roquette root tip cells
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Project
474-Yoshinari-NucleiAutofluoNIR
Dataset ID
MovS4_NIR_Roquette_RootTip_nuclei
Kind
Image data
Title
The time-lapse NIR autofluorescence imaging of nuclear dynamics in Roquette root tip cells
Description
The nuclei were observed to transform and migrate within roquette root tips cells of Eruca vesicaria subsp. Sativa. Time-lapse NIR autofluorescence images of nuclear dynamics in Roquette root tip cells were obtained. The NIR autofluorescence imaging approach has no need for a fluorescent protein reporter and can reduce the genetic manipulation time of introducing encoded nuclear markers into mutant plants to improve the efficiency of monitoring the cell nuclei movement in root.
Folder Name
MovS4_NIR_Roquette_RootTip_nuclei
File Format
czi
File Size
144MB
File Separation Method
-
Figure Number
MovieS4
Dimensions
1024x1024x1x2x72
X Scale
0.624 micrometer
Y Scale
0.624 micrometer
Z Scale
-
T Scale
20.26 sec
Channel
2 channel
Quantitative Data
-
Paper(s)

Biosample

Description
4-day-old Eruca vesicaria subsp. Sativa seedlings were incubated in liquid MGRL medium containing boric acid and sucrose.
Organism
Eruca vesicaria subsp. Sativa
(NCBITaxon:29727)
Strain
-
Cell
-
Cell line
-
MeSH
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)
Intrinsic Variable
-
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
-
Biological Process
nuclear migration (GO:0007097)
Cellular Component
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)
MeSH BP
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)
MeSH CC
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)
MeSH MF
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
-

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy
(FBbi:00000251)
fluorescence microscopy
(FBbi:00000246)
time lapse microscopy
(FBbi:00000249)
Detection Method
-
Visualization Method
NIR autofluorescence
Illumination Method
-
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
living tissue
MeSH
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)

Instrument

Body
Zeiss LSM800
Module
-
Light Source
The excitation wavelength was set at 640 nm with 0.90 mW at the back focal plane of the objective and the detection was set at 656–700 nm.
Detector
-
Objective
0.8 NA Plan-Apochromat, Zeiss, Oberkochen, Germany 1.1 NA LD C-Apochromat, Zeiss 1.4 NA Plan-Apochromat, Zeiss
Filter Set
-
Dichroic
-