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474-Yoshinari-NucleiAutofluoNIR/MovS3_NIR_CardaHir_GrowingRoot_nuclei thumbnail

474-Yoshinari-NucleiAutofluoNIR/MovS3_NIR_CardaHir_GrowingRoot_nuclei

Author reviewed
The time-lapse NIR autofluorescence imaging of root elongation in Cardamine hirsuta
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Project
474-Yoshinari-NucleiAutofluoNIR
Dataset ID
MovS3_NIR_CardaHir_GrowingRoot_nuclei
Kind
Image data
Title
The time-lapse NIR autofluorescence imaging of root elongation in Cardamine hirsuta
Description
The nuclear near-infrared (NIR) autofluorescence was observed more than 4 h in root elongation of 4-day-old Cardamine hirsuta. Time-lapse NIR autofluorescence images of root elongation were obtained. The NIR autofluorescence imaging approach has no need for a fluorescent protein reporter and can reduce the genetic manipulation time of introducing encoded nuclear markers into mutant plants to improve the efficiency of monitoring the cell nuclei movement in root.
Folder Name
MovS3_NIR_CardaHir_GrowingRoot_nuclei
File Format
czi
File Size
600MB
File Separation Method
-
Figure Number
MovieS3
Dimensions
1024x1024x1x2x300
X Scale
1.248 micrometer
Y Scale
1.248 micrometer
Z Scale
-
T Scale
59.80 sec
Channel
2 channel
Quantitative Data
-
Paper(s)

Biosample

Description
4-day-old Cardamine hirsuta seedlings were incubated in liquid MGRL medium containing boric acid and sucrose.
Organism
Cardamine hirsuta
(NCBITaxon:50463)
Strain
-
Cell
-
Cell line
-
MeSH
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)
Intrinsic Variable
-
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
-
Biological Process
root development (GO:0048364)
Cellular Component
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)
MeSH BP
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)
MeSH CC
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)
MeSH MF
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
-

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy
(FBbi:00000251)
fluorescence microscopy
(FBbi:00000246)
time lapse microscopy
(FBbi:00000249)
Detection Method
-
Visualization Method
NIR autofluorescence
Illumination Method
-
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
living tissue
MeSH
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)

Instrument

Body
Zeiss LSM800
Module
-
Light Source
The excitation wavelength was set at 640 nm with 0.90 mW at the back focal plane of the objective and the detection was set at 656–700 nm.
Detector
-
Objective
0.8 NA Plan-Apochromat, Zeiss, Oberkochen, Germany 1.1 NA LD C-Apochromat, Zeiss 1.4 NA Plan-Apochromat, Zeiss
Filter Set
-
Dichroic
-