SSBD:database

474-Yoshinari-NucleiAutofluoNIR/FigS2_NIR_H2B_Arabi_RootMeristem_EpiderCell_Mut thumbnail

474-Yoshinari-NucleiAutofluoNIR/FigS2_NIR_H2B_Arabi_RootMeristem_EpiderCell_Mut

Author reviewed
The time-lapse NIR autofluorescence images of epidermal cells in root meristemetic zone of mutant Arabidopsis expressing H2B-mClover
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Project
474-Yoshinari-NucleiAutofluoNIR
Dataset ID
FigS2_NIR_H2B_Arabi_RootMeristem_EpiderCell_Mut
Kind
Image data
Title
The time-lapse NIR autofluorescence images of epidermal cells in root meristemetic zone of mutant Arabidopsis expressing H2B-mClover
Description
The nuclear near-infrared (NIR) autofluorescence was observe the epidermal cells in root meristimetic zone of 4-day-old mutant Arabidopsis expressing H2B-mClover. Time-lapse NIR autofluorescence imaging visualized epidermal cells in the root meristematic zone of mutant Arabidopsis. The NIR autofluorescence imaging approach has no need for a fluorescent protein reporter and can reduce the genetic manipulation time of introducing encoded nuclear markers into mutant plants to improve the efficiency of monitoring the cell nuclei movement in root.
Folder Name
FigS2_NIR_H2B_Arabi_RootMeristem_EpiderCell_Mut
File Format
czi
File Size
900MB
File Separation Method
-
Figure Number
FigureS2
Dimensions
1024x1024x15x2x30
X Scale
0.312 micrometer
Y Scale
0.312 micrometer
Z Scale
1.5 micrometer
T Scale
600.01 sec
Channel
2 channel
Quantitative Data
-
Paper(s)

Biosample

Description
The 4-day-old trangenic Arabidopsis expressing H2B-mClover was grown on modified MGRL medium containing boric acid for 3 days.
Organism
Arabidopsis thaliana
(NCBITaxon:3702)
Strain
-
Cell
epidermal cell (CL:0000362)
Cell line
-
MeSH
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Meristem (D018519)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)
Intrinsic Variable
H2B–mClover was introduced into Arabidopsis thaliana by genetic transformation, resulting in transgenic plants expressing a histone H2B fused to the fluorescent protein mClover.
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
-
Biological Process
cell division (GO:0051301)
Cellular Component
nuclear envelope (GO:0005635)
nucleoplasm (GO:0005654)
Molecular Function
-
MeSH Anatomy
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Meristem (D018519)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)
MeSH BP
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Meristem (D018519)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)
MeSH CC
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Meristem (D018519)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)
MeSH MF
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Meristem (D018519)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)

Genetics

Gene
-
Protein / Probe
H2B
Method / Genotype
transgenic

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy
(FBbi:00000251)
fluorescence microscopy
(FBbi:00000246)
time lapse microscopy
(FBbi:00000249)
Detection Method
-
Visualization Method
H2B-mClover NIR autofluorescence
Illumination Method
-
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
living tissue
MeSH
Epidermal Cells (D000078404)
Microscopy, Confocal (D018613)
Meristem (D018519)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)

Instrument

Body
Zeiss LSM800
Module
-
Light Source
The excitation wavelength was set at 640 nm with 0.90 mW at the back focal plane of the objective and the detection was set at 656–700 nm.
Detector
-
Objective
0.8 NA Plan-Apochromat, Zeiss, Oberkochen, Germany 1.1 NA LD C-Apochromat, Zeiss 1.4 NA Plan-Apochromat, Zeiss
Filter Set
-
Dichroic
-