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474-Yoshinari-NucleiAutofluoNIR/Fig4c_NIR_Roquette_Root_nuclei thumbnail

474-Yoshinari-NucleiAutofluoNIR/Fig4c_NIR_Roquette_Root_nuclei

Author reviewed
The NIR depth-coding autofluorescence images of nuclei in Roquette roots
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Project
474-Yoshinari-NucleiAutofluoNIR
Dataset ID
Fig4c_NIR_Roquette_Root_nuclei
Kind
Image data
Title
The NIR depth-coding autofluorescence images of nuclei in Roquette roots
Description
A depth-coding image of nuclei based on z-stack images in roquette roots of 4-day-old Eruca vesicaria subsp. Sativa. The NIR autofluorescence imaging approach has no need for a fluorescent protein reporter and can reduce the genetic manipulation time of introducing encoded nuclear markers into mutant plants to improve the efficiency of monitoring the cell nuclei movement in root. Z-stack images were taken at different depths in a specimen and used to observe three dimensional structures.
Folder Name
Fig4c_NIR_Roquette_Root_nuclei
File Format
czi
File Size
132MB
File Separation Method
-
Figure Number
Figure4c
Dimensions
1024x1024x66x2x1
X Scale
0.624 micrometer
Y Scale
0.624 micrometer
Z Scale
2 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)

Biosample

Description
Eruca vesicaria was from home gardens. 4-day-old seedlings were incubated in liquid MGRL medium containing boric acid and sucrose.
Organism
Eruca vesicaria subsp. Sativa
(NCBITaxon:29727)
Strain
-
Cell
-
Cell line
-
MeSH
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Intrinsic Variable
-
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
-
Biological Process
nuclear migration (GO:0007097)
Cellular Component
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
MeSH BP
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
MeSH CC
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
MeSH MF
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
-

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy
(FBbi:00000251)
fluorescence microscopy
(FBbi:00000246)
Detection Method
-
Visualization Method
NIR autofluorescence
Illumination Method
-
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
living tissue
MeSH
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)

Instrument

Body
Zeiss LSM800
Module
-
Light Source
The excitation wavelength was set at 640 nm with 0.90 mW at the back focal plane of the objective and the detection was set at 656–700 nm.
Detector
-
Objective
0.8 NA Plan-Apochromat, Zeiss, Oberkochen, Germany 1.1 NA LD C-Apochromat, Zeiss 1.4 NA Plan-Apochromat, Zeiss
Filter Set
-
Dichroic
-