SSBD:database

474-Yoshinari-NucleiAutofluoNIR/Fig2d_NIR_H2B_Arabi_RootHair_nuclei_Mut thumbnail

474-Yoshinari-NucleiAutofluoNIR/Fig2d_NIR_H2B_Arabi_RootHair_nuclei_Mut

Author reviewed
The time-lapse NIR autofluorescence images of nuclear migration in mutant Arabidopsis expressing H2B-mClover
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Project
474-Yoshinari-NucleiAutofluoNIR
Dataset ID
Fig2d_NIR_H2B_Arabi_RootHair_nuclei_Mut
Kind
Image data
Title
The time-lapse NIR autofluorescence images of nuclear migration in mutant Arabidopsis expressing H2B-mClover
Description
The nuclear migration towards the apical or basal side in mutant Arabidopsis root hair cells expressing H2B-mClover, which is a fluorescently tagged version of the histone protain H2B. Time-lapse NIR autofluorescence imaging revealed the nuclear migration in mutant Arabidopsis root hair cells. The NIR autofluorescence imaging approach has no need for a fluorescent protein reporter and can reduce the genetic manipulation time of introducing encoded nuclear markers into mutant plants to improve the efficiency of monitoring the cell nuclei movement in root.
Folder Name
Fig2d_NIR_H2B_Arabi_RootHair_nuclei_Mut
File Format
czi
File Size
33MB
File Separation Method
-
Figure Number
Figure2d
Dimensions
1024x1024x1x3x11
X Scale
0.312 micrometer
Y Scale
0.312 micrometer
Z Scale
-
T Scale
300.34 sec
Channel
3 channel
Quantitative Data
-
Paper(s)

Biosample

Description
Arabidopsis plants expressing H2B-mClover were provided by other research group. 4-day-old seedlings were incubated in liquid MGRL medium containing boric acid and sucrose for 4 days.
Organism
Arabidopsis thaliana
(NCBITaxon:3702)
Strain
-
Cell
-
Cell line
-
MeSH
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)
Intrinsic Variable
H2B–mClover was introduced into Arabidopsis thaliana by genetic transformation, resulting in transgenic plants expressing a histone H2B fused to the fluorescent protein mClover.
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
-
Biological Process
nuclear migration (GO:0007097)
Cellular Component
nucleus (GO:0005634)
root hair (GO:0035618)
Molecular Function
-
MeSH Anatomy
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)
MeSH BP
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)
MeSH CC
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)
MeSH MF
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)

Genetics

Gene
-
Protein / Probe
H2B
Method / Genotype
transgenic

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy
(FBbi:00000251)
fluorescence microscopy
(FBbi:00000246)
time lapse microscopy
(FBbi:00000249)
Detection Method
-
Visualization Method
H2B-mClover NIR autofluorescence
Illumination Method
-
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
living tissue
MeSH
Microscopy, Confocal (D018613)
Cell Nucleus (NAD002467)
Microscopy, Fluorescence (D008856)
Plant Roots (D018517)
Time-Lapse Imaging (D059008)

Instrument

Body
Zeiss LSM800
Module
-
Light Source
The excitation wavelength was set at 640 nm with 0.90 mW at the back focal plane of the objective and the detection was set at 656–700 nm.
Detector
-
Objective
0.8 NA Plan-Apochromat, Zeiss, Oberkochen, Germany 1.1 NA LD C-Apochromat, Zeiss 1.4 NA Plan-Apochromat, Zeiss
Filter Set
-
Dichroic
-