SSBD:database

474-Yoshinari-NucleiAutofluoNIR/Fig2c_NIR_SYBR_Arabi_Pollen_nucleus_WT thumbnail

474-Yoshinari-NucleiAutofluoNIR/Fig2c_NIR_SYBR_Arabi_Pollen_nucleus_WT

Author reviewed
The time-lapse NIR autofluorescence and SYBR Green I images of vegetative nucleus during pollen tube elongation in wild-type Arabidopsis
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Project
474-Yoshinari-NucleiAutofluoNIR
Dataset ID
Fig2c_NIR_SYBR_Arabi_Pollen_nucleus_WT
Kind
Image data
Title
The time-lapse NIR autofluorescence and SYBR Green I images of vegetative nucleus during pollen tube elongation in wild-type Arabidopsis
Description
The DNA of wild-type Arabidopsis Col-0 was co-stained with SYBR Green I, a green fluorescent dye, to measure nuclear near-infrared (NIR) autofluorescence in pollen. Time-lapse NIR autofluorescence imaging revealed the dynamics of the vegetative nucleus during pollen tube elongation in wild-type Arabidopsis. The NIR autofluorescence promoted clearer imaging of the vegetative nucleus than DNA dyes during pollen tube elongation.
Folder Name
Fig2c_NIR_SYBR_Arabi_Pollen_nucleus_WT
File Format
czi
File Size
91MB
File Separation Method
-
Figure Number
Figure2c
Dimensions
512x512x1x5x73
X Scale
0.198 micrometer
Y Scale
0.198 micrometer
Z Scale
-
T Scale
14.90 sec
Channel
5 channel
Quantitative Data
-
Paper(s)

Biosample

Description
Pollen grains were collected from Col-0 plants and germinated. The pollen germination was stained by medium containing SYBR Green I.
Organism
Arabidopsis thaliana
(NCBITaxon:3702)
Strain
-
Cell
-
Cell line
-
MeSH
Microscopy, Confocal (D018613)
Pollen Tube (D053205)
Microscopy, Fluorescence (D008856)
Time-Lapse Imaging (D059008)
Intrinsic Variable
-
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
-
Biological Process
pollen tube development (GO:0048868)
Cellular Component
pollen tube (GO:0090406)
Molecular Function
-
MeSH Anatomy
Microscopy, Confocal (D018613)
Pollen Tube (D053205)
Microscopy, Fluorescence (D008856)
Time-Lapse Imaging (D059008)
MeSH BP
Microscopy, Confocal (D018613)
Pollen Tube (D053205)
Microscopy, Fluorescence (D008856)
Time-Lapse Imaging (D059008)
MeSH CC
Microscopy, Confocal (D018613)
Pollen Tube (D053205)
Microscopy, Fluorescence (D008856)
Time-Lapse Imaging (D059008)
MeSH MF
Microscopy, Confocal (D018613)
Pollen Tube (D053205)
Microscopy, Fluorescence (D008856)
Time-Lapse Imaging (D059008)

Genetics

Gene
-
Protein / Probe
-SYBR Green I
Method / Genotype
-

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
confocal microscopy
(FBbi:00000251)
fluorescence microscopy
(FBbi:00000246)
time lapse microscopy
(FBbi:00000249)
Detection Method
-
Visualization Method
SYBR Green I NIR autofluorescence
Illumination Method
-
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
living tissue
MeSH
Microscopy, Confocal (D018613)
Pollen Tube (D053205)
Microscopy, Fluorescence (D008856)
Time-Lapse Imaging (D059008)

Instrument

Body
Zeiss LSM800
Module
-
Light Source
The excitation wavelength was set at 640 nm with 0.90 mW at the back focal plane of the objective and the detection was set at 656–700 nm.
Detector
-
Objective
0.8 NA Plan-Apochromat, Zeiss, Oberkochen, Germany 1.1 NA LD C-Apochromat, Zeiss 1.4 NA Plan-Apochromat, Zeiss 63× oil-immersion objective lens
Filter Set
-
Dichroic
-