473-Louder-ZebraFinch/FigS4A_Nidopallium_Right_GFP
Author reviewedZ-series images of a cleared right brain of zebra finch showing tutor’s song responsive neurons in NCM
Project
Dataset ID
FigS4A_Nidopallium_Right_GFP
Kind
Image data
Title
Z-series images of a cleared right brain of zebra finch showing tutor’s song responsive neurons in NCM
Description
Z-series images obtained with a custom-made light-sheet fluorescence microscope. The EYFP expressing neurons were immunohistochemically enhanced with anti-GFP antibody.
Folder Name
FigS4A_Nidopallium_Right_GFP
File Format
.tif
File Size
18.8GB
File Separation Method
The GFP images and propidium iodide images are separated into different datasets
Figure Number
FigS4A
Dimensions
2160x2560x1699x1x1
X Scale
5.2 micrometer
Y Scale
5.2 micrometer
Z Scale
5.2 micrometer
T Scale
-
Channel
1 channel
Quantitative Data
-
Paper(s)
Biosample
Description
Fixed and cleared zebra finch brain after song learning from the tutor-bird. To specifically express EYFP in the caudomedial nidopallium (NCM) neurons activated by hearing the tutor’s song, the authors used Adeno-associated viral vector with a cFos promotor and tetracycline (TetON) system. The Zebra finch adult injected with the AAV-cFos-TetON-EYFP-PEST in the NCM were exposed to tutor’s song playback concurrent with doxycycline in their drinking water to induce EYFP expression only in tutor’s song-responsive neurons.
Organism
Taeniopygia guttata (NCBITaxon:59729)
Strain
-
Cell
neuron (CL:0000540)
Cell line
-
Intrinsic Variable
-
Extrinsic Variable
Adeno-associated viral vector, AAV-cFos-TetON-EYFP-PEST, was injected to the bird until at least 60 days post-hatching.
Ontology
Anatomical Entity
-
UBERON
brain (UBERON:0000955)
Biological Process
regulation of nervous system development (GO:0051960)
Cellular Component
cytoplasm (GO:0005737)
Molecular Function
-
Genetics
Gene
-
Protein / Probe
Green fluorescent protein / antibody
Method / Genotype
injection
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
light-sheet microscopy
Detection Method
-
Visualization Method
primary antibody plus labeled secondary antibody
Illumination Method
light-sheet illumination
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
whole mounted tissue
Instrument
Body
Olympus-Evident MVX10
Module
Custum Build Light-Sheet
Light Source
Coherent OBIS 488 nm, 100 mW
Detector
PCO edge 5.5
Objective
Olympus-Evident MVPLAPO0.63X
Filter Set
Semrock FF01-536/40-32
Dichroic
-