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460-Tanaka-RadialAxisDyn/Fig3B_hdg11_12pdf2

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The time-lapse images of observing in vitro-cultivated hdg11/12 pdf2 ovules expressing WOX2p::H2B-GFP and WOX2p::LTI-tdTomato

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Project

460-Tanaka-RadialAxisDyn

Dataset ID

Fig3B_hdg11_12pdf2

Kind

Image data

Title

The time-lapse images of observing in vitro-cultivated hdg11/12 pdf2 ovules expressing WOX2p::H2B-GFP and WOX2p::LTI-tdTomato

Description

The marker WOX2p::H2B-GFP to label the embryo nucleus of Arabidopsis and the marker WOX2p::LTI-tdTomato to label the plasma membrane (PM) showed that after the zygote division the apical cell of in vitro-cultivated hdg11/12 pdf2 ovules expands to take on a special shape before dividing vertically. The pdf2 mutant was introduced into the hdg11/12 mutant to get the hdg11/12 pdf2 triple mutant.

Folder Name

Fig3B_hdg11_12pdf2

File Format

.tif

File Size

2.95GB

File Separation Method

Figure Number

Figure3B

Dimensions

512x512x11x2x256

X Scale

2.0 micrometer

Y Scale

2.0 micrometer

Z Scale

5.0 micrometer

T Scale

10 minutes

Channel

2 channel

Quantitative Data

Paper(s)

Biosample

Description

The embryo nucleus of Arabidopsis vitro-cultivated hdg11/12 pdf2 ovules was labelld by the markers WOX2p::H2B-GFP and WOX2p::LTI-tdTomato.

Organism

Arabidopsis thaliana

(NCBITaxon:3702)

Strain

Col-0 (https://abrc.osu.edu/stocks/1)

Cell

zygote (CL:0010017)

Cell line

MeSH

Zygote (D015053)

Microscopy, Confocal (D018613)

Cell Nucleus (D002467)

Cell Membrane (D002462)

Intrinsic Variable

The marker WOX2p::H2B-GFP was to label the embryo nucleus, and the marker WOX2p::LTI-tdTomato was to label the plasma membrane (PM) of Arabidopsis. In the hdg11/12 pdf2 ovules, HDG11 (homeodomain glabrous11), HDG12 (homeodomain glabrous12) and PDF2 (protodermal factor2 ) lose their function by transfer DNA (T-DNA) insertion.

Extrinsic Variable

Ontology

Anatomical Entity

UBERON

zygote stage (UBERON:0000106)

Biological Process

zygote elongation (GO:0080159)

Cellular Component

nucleus (GO:0005634)

plasma membrane (GO:0005886)

Molecular Function

MeSH Anatomy

Zygote (D015053)

Microscopy, Confocal (D018613)

Cell Nucleus (D002467)

Cell Membrane (D002462)

MeSH BP

Zygote (D015053)

Microscopy, Confocal (D018613)

Cell Nucleus (D002467)

Cell Membrane (D002462)

MeSH CC

Zygote (D015053)

Microscopy, Confocal (D018613)

Cell Nucleus (D002467)

Cell Membrane (D002462)

MeSH MF

Zygote (D015053)

Microscopy, Confocal (D018613)

Cell Nucleus (D002467)

Cell Membrane (D002462)

Genetics

Gene

Protein / Probe

H2B

Method / Genotype

transgenic

Gene

Protein / Probe

Hydrophobic protein RCI2B

Method / Genotype

transgenic

Gene

HDG11

Protein / Probe

Method / Genotype

transgenic

Gene

HDG12

Protein / Probe

Method / Genotype

transgenic

Gene

PDF2

Protein / Probe

Method / Genotype

transgenic

Treatments

Reagent / Compound

Concentration

Fold Dilution

Imaging Method

spinning disk confocal microscopy

(FBbi:00000253)

Detection Method

spot detector

Visualization Method

Green fluorescent protein from Aequorea tdTomatoFP

Illumination Method

Source of Contrast

Imaged Parameter

fluorescence emission

Sample Preparation Method

living tissue

MeSH

Zygote (D015053)

Microscopy, Confocal (D018613)

Cell Nucleus (D002467)

Cell Membrane (D002462)

Instrument

Body

Yokogawa Electric CSU-W1

Module

Light Source

Detector

Objective

Filter Set

Dichroic