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460-Tanaka-RadialAxisDyn/Fig3A_WT thumbnail

460-Tanaka-RadialAxisDyn/Fig3A_WT

Author reviewed
The time-lapse images of observing in vitro-cultivated WT ovules expressing WOX2p::H2B-GFP and WOX2p::LTI-tdTomato
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Project
460-Tanaka-RadialAxisDyn
Dataset ID
Fig3A_WT
Kind
Image data
Title
The time-lapse images of observing in vitro-cultivated WT ovules expressing WOX2p::H2B-GFP and WOX2p::LTI-tdTomato
Description
The marker WOX2p::H2B-GFP to label the embryo nucleus of Arabidopsis and the marker WOX2p::LTI-tdTomato to label the plasma membrane (PM) showed that after the zygote division the apical cell of in vitro-cultivated WT ovules expands to take on a special shape before dividing vertically. The Arabidopsis lines expressing WOX2::H2B-GFP and WOX2p::LTI-tdTomato were original to the authors of this paper.
Folder Name
Fig3A_WT
File Format
.tif
File Size
2GB
File Separation Method
-
Figure Number
Figure3A
Dimensions
512x512x11x2x173
X Scale
2.0 micrometer
Y Scale
2.0 micrometer
Z Scale
5.0 micrometer
T Scale
10 minutes
Channel
2 channel
Quantitative Data
-
Paper(s)

Biosample

Description
The embryo nucleus of Arabidopsis vitro-cultivated WT ovules was labelld by the markers WOX2p::H2B-GFP and WOX2p::LTI-tdTomato.
Organism
Arabidopsis thaliana
(NCBITaxon:3702)
Strain
Col-0 (https://abrc.osu.edu/stocks/1)
Cell
zygote (CL:0010017)
Cell line
-
MeSH
Zygote (D015053)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)
Cell Membrane (D002462)
Intrinsic Variable
The marker WOX2p::H2B-GFP was to label the embryo nucleus, and the marker WOX2p::LTI-tdTomato was to label the plasma membrane (PM) of Arabidopsis.
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
zygote stage (UBERON:0000106)
Biological Process
zygote elongation (GO:0080159)
Cellular Component
nucleus (GO:0005634)
plasma membrane (GO:0005886)
Molecular Function
-
MeSH Anatomy
Zygote (D015053)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)
Cell Membrane (D002462)
MeSH BP
Zygote (D015053)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)
Cell Membrane (D002462)
MeSH CC
Zygote (D015053)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)
Cell Membrane (D002462)
MeSH MF
Zygote (D015053)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)
Cell Membrane (D002462)

Genetics

Gene
-
Protein / Probe
H2B
Method / Genotype
transgenic
Gene
-
Protein / Probe
Hydrophobic protein RCI2B
Method / Genotype
transgenic

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
spinning disk confocal microscopy
(FBbi:00000253)
Detection Method
spot detector
Visualization Method
Green fluorescent protein from Aequorea tdTomatoFP
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Zygote (D015053)
Microscopy, Confocal (D018613)
Cell Nucleus (D002467)
Cell Membrane (D002462)

Instrument

Body
Yokogawa Electric CSU-W1
Module
-
Light Source
-
Detector
-
Objective
-
Filter Set
-
Dichroic
-