456-Ezaki-FESEMCellProlife/Fig3A_5dasLeafPrimordia_an3_04
Author reviewedZ-series images of 5 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample4 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Project
Dataset ID
Fig3A_5dasLeafPrimordia_an3_04
Kind
Image data
Title
Z-series images of 5 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample4 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Description
The 5 DAS leaf primordia of an3 mutant-Arabidopsis thaliana sample4 stained with BCECF-AM, used as the fluorescent indicator for vacuole, and FM4-64, used as the dye for plasma membrane. Z-series images were obtained by confocal microscopy.
The size and shape of vacuole in the mutant an3 were larger and more unified.
The ANGUSTIFOLIA (AN3) gene is a positive regulator of cell proliferation and an3-4 allele was isolated by Horiguchi et al. (2005).
Folder Name
Fig3A_5dasLeafPrimordia_an3_04
File Format
oir
File Size
224MB
File Separation Method
-
Figure Number
Figure3A
Dimensions
1024x1024x56x2x1
X Scale
0.124 micrometer
Y Scale
0.124 micrometer
Z Scale
0.42 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
The leaf primordia was cut from a 5 DAS an3 mutant-Arabidopsis thaliana seedling. The vacuoles were stained with BCECF-AM. The plasma membranes were stained with FM4-64.
Organism
Arabidopsis thaliana
Strain
an3-4
Cell
vacuole
plasma membrane (GO:0005886)
Cell line
plasma membrane (GO:0005886)
MeSH
Intrinsic Variable
In the an3-4 strain, ANGUSTIFOLIA (AN3) gene is mutated. It was identified by screening for leaf-shape mutants in M2 populations generated by fast neutron bombardment and X-ray irradiation.
Extrinsic Variable
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Ontology
Anatomical Entity
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UBERON
primordium (UBERON:0001048)
Biological Process
cell population proliferation (GO:0008283)
cell differentiation (GO:0030154)
cell division (GO:0051301)
Cellular Component
vacuole (GO:0005773)
plasma membrane (GO:0005886)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
AN3
Protein / Probe
-
Method / Genotype
radiation induced mutation
Gene
-
Protein / Probe
-FM4-64
Method / Genotype
-
Gene
-
Protein / Probe
-BCECF-AM
Method / Genotype
-
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy
Detection Method
-
Visualization Method
fluorescent label
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Olympus-Evident FV3000
Module
-
Light Source
BCECF was excited with a laser at 488 nm, and the emission was detected between 500 and 570 nm.
For FM4-64, the excitation wavelength was 514 nm, and emission was detected between 600 and 640 nm.
Detector
-
Objective
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Filter Set
-
Dichroic
-