456-Ezaki-FESEMCellProlife/Fig2BC_FE-SEM_WT_2_10_x6000_70 thumbnail

456-Ezaki-FESEMCellProlife/Fig2BC_FE-SEM_WT_2_10_x6000_70

Author reviewed
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample2
Project
Dataset ID
Fig2BC_FE-SEM_WT_2_10_x6000_70
Kind
Image data
Title
FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of WT-Arabidopsis thaliana_sample2
Description
The adaxial subepidermal cells in 4 DAS (Days After Sowing) leaf primordia cross-sections of wild-type (WT) Arabidopsis thaliana sample2 were observed using a field emission-scanning electron microscope (FE-SEM). Compared to mutant an3, small vacuoles and much less intercellular space were shown in WT. The image of chloroplast was magnified from the image of adaxial subepidermal cells.
Folder Name
Fig2BC_FE-SEM_WT_2_10_x6000_70
File Format
tif
File Size
19MB
File Separation Method
-
Figure Number
Figure2B_2C
Dimensions
5120x3840x1x1x1
X Scale
0.0042 micrometer
Y Scale
0.0042 micrometer
Z Scale
-
T Scale
-
Channel
-
Quantitative Data
-

Paper(s)

Biosample

Description
The leaf primordia was cut from a 4 DAS WT-Arabidopsis thaliana seedling, fixed with reagents. The 1 micrometer cross-sections of leaf primordia on a glass slide were stained.
Organism
Arabidopsis thaliana
Strain
Cell
adaxial subepidermal cells
Cell line
-
MeSH
Chloroplasts (D002736)
Microscopy, Electron, Scanning (D008855)
Vacuoles (D014617)
Thylakoids (D020524)
Intrinsic Variable
-
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
primordium (UBERON:0001048)
Biological Process
-
Cellular Component
vacuole (GO:0005773)
chloroplast (GO:0009507)
thylakoid membrane (GO:0042651)
Molecular Function
-
MeSH Anatomy
Chloroplasts (D002736)
Microscopy, Electron, Scanning (D008855)
Vacuoles (D014617)
Thylakoids (D020524)
MeSH BP
Chloroplasts (D002736)
Microscopy, Electron, Scanning (D008855)
Vacuoles (D014617)
Thylakoids (D020524)
MeSH CC
Chloroplasts (D002736)
Microscopy, Electron, Scanning (D008855)
Vacuoles (D014617)
Thylakoids (D020524)
MeSH MF
Chloroplasts (D002736)
Microscopy, Electron, Scanning (D008855)
Vacuoles (D014617)
Thylakoids (D020524)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
-

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
scanning electron microscopy (SEM)
(FBbi:00000257)
Detection Method
-
Visualization Method
electron-dense stain
Illumination Method
-
Source of Contrast
-
Imaged Parameter
electron density
Sample Preparation Method
sectioned tissue
MeSH
Chloroplasts (D002736)
Microscopy, Electron, Scanning (D008855)
Vacuoles (D014617)
Thylakoids (D020524)

Instrument

Body
Hitachi-FE-SEM SU8200
Module
-
Light Source
-
Detector
-
Objective
-
Filter Set
-
Dichroic
-