SSBD:database

Project

Dataset ID

FE-SEM_an3_3_8_x6000

Kind

Image data

Title

FE-SEM image of adaxial subepidermal cells and chloroplast in 4 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample3

Description

The adaxial subepidermal cells in 4 DAS leaf primordia cross-sections of an3 mutant-Arabidopsis thaliana sample3 were observed using a field emission-scanning electron microscope (FE-SEM). Compared to WT, larger vacuoles and extended intercellular space were exhibited in mutant an3. The chloroplast with more thylakoid membranes in the mutant an3 were observed using a field emission-scanning electron microscope (FE-SEM), compared to that of WT. The image of chloroplast was magnified from the image of adaxial subepidermal cells. The ANGUSTIFOLIA (AN3) gene is a positive regulator of cell proliferation and an3-4 allele was isolated by Horiguchi et al. (2005). Other mutant an3 alleles were provided by multiple research groups and introgressed into Col-0.

Folder Name

FE-SEM_an3_3_8_x6000

File Format

tif

File Size

19MB

File Separation Method

-

Figure Number

Figure2B_2C

Dimensions

5120x3840x1x1x1

X Scale

0.0042 micrometer

Y Scale

0.0042 micrometer

Z Scale

-

T Scale

-

Channel

-

Quantitative Data

-

Description

The leaf primordia was cut from a 4 DAS an3 mutant-Arabidopsis thaliana seedling, fixed with reagents. The 1 micrometer cross-sections of leaf primordia on a glass slide were stained.

Organism

Strain

an3-4

Cell

adaxial subepidermal cells

Cell line

-

MeSH

Intrinsic Variable

In the an3-4 strain, ANGUSTIFOLIA (AN3) gene is mutated. It was identified by screening for leaf-shape mutants in M2 populations generated by fast neutron bombardment and X-ray irradiation.

Extrinsic Variable

-

Anatomical Entity

-

UBERON

Biological Process

-

Cellular Component

Molecular Function

-

MeSH Anatomy

MeSH BP

MeSH CC

MeSH MF

Imaging Method

Detection Method

-

Visualization Method

electron-dense stain

Illumination Method

-

Source of Contrast

-

Imaged Parameter

electron density

Sample Preparation Method

sectioned tissue

MeSH

Body

Hitachi-FE-SEM SU8200

Module

-

Light Source

-

Detector

-

Objective

-

Filter Set

-

Dichroic

-