454-Mizuta-PollenTubeGuidance/Fig6A_PollenTube_gcs1
Author reviewedZ-series images of pollen tube in gcs1 mutant Arabidopsis
Dataset ID
Fig6A_PollenTube_gcs1
Kind
Image data
Title
Z-series images of pollen tube in gcs1 mutant Arabidopsis
Description
Two-photon z-series images of the gcs1 (GENERATIVE CELL-SPECIFIC 1) mutant Arabidopsis thaliana pistil in its pollen tube. Pollen tubes in the pistil were stained with aniline blue in the NaOH-clearing solution.
Folder Name
Fig6A_PollenTube_gcs1
File Format
.nd2
File Size
52MB
File Separation Method
-
Figure Number
Fig6A
Dimensions
512x512x52x2x1
X Scale
1 micrometer
Y Scale
1 micrometer
Z Scale
2 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
The dissected pistil of gcs1 mutant Arabidopsis after hand pollination.
Organism
Arabidopsis thaliana (NCBITaxon:3702)
Strain
Cell
ovary (UBERON:0000992)
Cell line
-
MeSH
Intrinsic Variable
The mutant seeds of gcs1 mutant (SALK_135496) was used.
Extrinsic Variable
-
Ontology
Anatomical Entity
pollen tube (EFO:0001004)
pistil (EFO:0001004)
UBERON
ovary (UBERON:0000992)
Biological Process
pollen tube guidance (GO:0010183)
Cellular Component
cytoplasm (GO:0005737)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-Fluorescent dye (Aniline blue)
Method / Genotype
staining
clearing
Gene
HAP2
Protein / Probe
-
Method / Genotype
transgenic / gcs1 (SALK_135496)
Treatments
Reagent / Compound
Aniline blue
Concentration
-
Fold Dilution
0.1 percent
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
autofluorescence
aniline blue
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
fixation method, tissue clearing, Staining
MeSH
Instrument
Body
Nikon A1RMP
Module
-
Light Source
Spectra-Physics Mai Tai DeepSee
Detector
Nikon non-descanned GaAsP PMT
detectors
Objective
Nikon CFI75 Apo 25xW MP, WD = 2 mm;
NA = 1.10
Filter Set
479/40 nm for mTFP1
578/105 nm for TagRFP
Dichroic
DM495LP
DM560LP