454-Mizuta-PollenTubeGuidance/Fig5A_PollenTube_WT
Author reviewedZ-series images of pollen tube expressing mTFP1 in WT Arabidopsis
Dataset ID
Fig5A_PollenTube_WT
Kind
Image data
Title
Z-series images of pollen tube expressing mTFP1 in WT Arabidopsis
Description
Two-photon z-series images of the wild type Arabidopsis thaliana pistil expressing mTFP1 in its pollen tube. The pollen tubes were visualized with autofluorescence and mTFP1 fluorescence.
Folder Name
Fig5A_PollenTube_WT
File Format
.nd2
File Size
221MB
File Separation Method
-
Figure Number
Fig5A
Dimensions
512x2459x46x2x1
X Scale
1 micrometer
Y Scale
1 micrometer
Z Scale
7 micrometer
T Scale
-
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
The dissected pistil of wild type Arabidopsis after hand pollination. The sample was fixed and cleared by using ClearSee (plant clearing solution).
Organism
Arabidopsis thaliana (NCBITaxon:3702)
Strain
Cell
ovary (UBERON:0000992)
Cell line
-
MeSH
Intrinsic Variable
LAT52p::mTFP1 was expressed in the pollen tube.
Extrinsic Variable
-
Ontology
Anatomical Entity
pollen tube (EFO:0001004)
pistil (EFO:0001004)
UBERON
ovary (UBERON:0000992)
Biological Process
pollen tube guidance (GO:0010183)
Cellular Component
cytoplasm (GO:0005737)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transgenic clearing / LAT52p::mTFP1
Treatments
Reagent / Compound
ClearSee (plant clearing solution)
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
mTFP1 autofluorescence
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
formaldehyde fixed tissue, tissue clearing
MeSH
Instrument
Body
Nikon A1RMP
Module
-
Light Source
Spectra-Physics Mai Tai DeepSee
Detector
Nikon non-descanned GaAsP PMT
detectors
Objective
Nikon CFI75 Apo 25xW MP, WD = 2 mm;
NA = 1.10
Filter Set
479/40 nm for mTFP1
578/105 nm for TagRFP
Dichroic
DM495LP
DM560LP