454-Mizuta-PollenTubeGuidance/EV3B_ferMutant_polytuby
Author reviewedPollen tube emergence and polytubey in the fer pistil
Dataset ID
EV3B_ferMutant_polytuby
Kind
Image data
Title
Pollen tube emergence and polytubey in the fer pistil
Description
Two-photon z-series and time-lapse images of the fer (FERONIA) mutant Arabidopsis thaliana pistil expressing mApple in its pollen tube. Nuclei in the septal epidermal cells were labeled with YFP.
Folder Name
EV3B_ferMutant_polytuby
File Format
.nd2
File Size
1.3GB
File Separation Method
-
Figure Number
Fig7B
Dimensions
512x512x20x2x65
X Scale
0.503 micrometer
Y Scale
0.503 micrometer
Z Scale
8 micrometer
T Scale
15 minutes
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
The dissected pistil of fer mutant Arabidopsis after hand pollination.
Organism
Arabidopsis thaliana (NCBITaxon:3702)
Strain
Cell
ovary (UBERON:0000992)
Cell line
-
MeSH
Intrinsic Variable
LAT52p::mApple was expressed in the pollen tube.
The mutant seeds of fer (fer-4; GK-106A06) was used.
Extrinsic Variable
-
Ontology
Anatomical Entity
pollen tube (EFO:0001004)
pistil (EFO:0001004)
UBERON
ovary (UBERON:0000992)
Biological Process
pollen tube guidance (GO:0010183)
Cellular Component
cytoplasm (GO:0005737)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transgenic / LAT52p::mApple
Gene
FER
Protein / Probe
-
Method / Genotype
transgenic / fer (fer-4; GK-106A06)
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
mAppleFP autofluorescence
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Nikon A1RMP
Module
-
Light Source
Spectra-Physics Mai Tai DeepSee
Detector
Nikon non-descanned GaAsP PMT
detectors
Objective
Nikon CFI75 Apo 25xW MP, WD = 2 mm;
NA = 1.10
Filter Set
534/30 nm for GFP
578/105 nm for TagRFP
Dichroic
DM495LP
DM560LP