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454-Mizuta-PollenTubeGuidance/EV3A_PollenTube_Epiderm thumbnail

454-Mizuta-PollenTubeGuidance/EV3A_PollenTube_Epiderm

Author reviewed
Pollen tube emergence in the HDG11p::NLS-YFP pistil
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Project
454-Mizuta-PollenTubeGuidance
Dataset ID
EV3A_PollenTube_Epiderm
Kind
Image data
Title
Pollen tube emergence in the HDG11p::NLS-YFP pistil
Description
Two-photon z-series and time-lapse images of the wild type Arabidopsis thaliana pistil expressing mApple in its pollen tube. Nuclei in the septal epidermal cells were labeled with YFP.
Folder Name
EV3A_PollenTube_Epiderm
File Format
.nd2
File Size
1.7GB
File Separation Method
-
Figure Number
Fig7A
Dimensions
512x512x21x2x82
X Scale
1 micrometer
Y Scale
1 micrometer
Z Scale
7 micrometer
T Scale
15 minutes
Channel
2 channel
Quantitative Data
-
Paper(s)

Biosample

Description
The dissected pistil of HDG11p::NLS-YFP Arabidopsis after hand pollination.
Organism
Arabidopsis thaliana (NCBITaxon:3702)
Strain
Col-0 (https://arapheno.1001genomes.org/accession/6909/)
Cell
ovary epidermal cell (CL:0000362)
Cell line
-
MeSH
Ovary (D010053)
Microscopy, Fluorescence, Multiphoton (D018613)
Pollen Tube (D053205)
Cytoplasm (D003593)
Epidermal Cells (D000078404)
Time-Lapse Imaging (D059008)
Cell Nucleus (D002467)
Intrinsic Variable
LAT52p::mApple was expressed in the pollen tube. Nuclei in the septal epidermal cells of HDG11p::NLS-YFP are labeled with YFP.
Extrinsic Variable
-

Ontology

Anatomical Entity
pollen tube (EFO:0001004)
pistil (EFO:0001004)
UBERON
ovary (UBERON:0000992)
Biological Process
pollen tube guidance (GO:0010183)
Cellular Component
cytoplasm (GO:0005737)
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
Ovary (D010053)
Microscopy, Fluorescence, Multiphoton (D018613)
Pollen Tube (D053205)
Cytoplasm (D003593)
Epidermal Cells (D000078404)
Time-Lapse Imaging (D059008)
Cell Nucleus (D002467)
MeSH BP
Ovary (D010053)
Microscopy, Fluorescence, Multiphoton (D018613)
Pollen Tube (D053205)
Cytoplasm (D003593)
Epidermal Cells (D000078404)
Time-Lapse Imaging (D059008)
Cell Nucleus (D002467)
MeSH CC
Ovary (D010053)
Microscopy, Fluorescence, Multiphoton (D018613)
Pollen Tube (D053205)
Cytoplasm (D003593)
Epidermal Cells (D000078404)
Time-Lapse Imaging (D059008)
Cell Nucleus (D002467)
MeSH MF
Ovary (D010053)
Microscopy, Fluorescence, Multiphoton (D018613)
Pollen Tube (D053205)
Cytoplasm (D003593)
Epidermal Cells (D000078404)
Time-Lapse Imaging (D059008)
Cell Nucleus (D002467)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transgenic / HDG11p::NLS-YFP
Gene
-
Protein / Probe
-
Method / Genotype
transgenic / LAT52p::mApple

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
YFP mAppleFP autofluorescence
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Ovary (D010053)
Microscopy, Fluorescence, Multiphoton (D018613)
Pollen Tube (D053205)
Cytoplasm (D003593)
Epidermal Cells (D000078404)
Time-Lapse Imaging (D059008)
Cell Nucleus (D002467)

Instrument

Body
Nikon A1RMP
Module
-
Light Source
Spectra-Physics Mai Tai DeepSee
Detector
Nikon non-descanned GaAsP PMT detectors
Objective
Nikon CFI75 Apo 25xW MP, WD = 2 mm; NA = 1.10
Filter Set
534/30 nm for GFP 578/105 nm for TagRFP
Dichroic
DM495LP DM560LP