454-Mizuta-PollenTubeGuidance/EV1C_PollenTube_Synergid
Author reviewedPollen tube dynamics in the Arabidopsis MYB98p::GFP pistil
Dataset ID
EV1C_PollenTube_Synergid
Kind
Image data
Title
Pollen tube dynamics in the Arabidopsis MYB98p::GFP pistil
Description
Time-lapse images of the pistil of Arabidopsis thaliana. Pollen tube growth and pollen tube guidance within the pistil are visualized by expressing mTFP1 or TagRFP. Synergid cells were visualized with GFP.
Folder Name
EV1C_PollenTube_Synergid
File Format
.nd2
File Size
4.3MB
File Separation Method
-
Figure Number
Fig1DEF
Dimensions
245x234x1x3x13
X Scale
1 micrometer
Y Scale
1 micrometer
Z Scale
-
T Scale
15 minutes
Channel
3 channel
Quantitative Data
-
Paper(s)
Biosample
Description
The dissected pistil of MYB98p::GFP Arabidopsis after hand pollination.
Organism
Arabidopsis thaliana (NCBITaxon:3702)
Strain
Cell
ovary
synergid cell (BTO:0006121)
Cell line
-
MeSH
Intrinsic Variable
LAT52p::mTFP1 and LAT52p::TagRFP were expressed in the pollen tube. MYB98p::GFP was expressed in the synergid cell.
Extrinsic Variable
-
Ontology
Anatomical Entity
pollen tube (EFO:0001004)
pistil (EFO:0001004)
UBERON
ovary (UBERON:0000992)
Biological Process
pollen tube guidance (GO:0010183)
Cellular Component
cytoplasm (GO:0005737)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transgenic / LAT52p::mTFP1
Gene
-
Protein / Probe
-
Method / Genotype
transgenic / LAT52p::TagRFP
Gene
-
Protein / Probe
-
Method / Genotype
transgenic / MYB98p::GFP
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
mTFP1
TagRFP
GFP
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Nikon A1RMP
Module
-
Light Source
Spectra-Physics Mai Tai DeepSee
Detector
Nikon non-descanned GaAsP PMT
detectors
Objective
Nikon CFI75 Apo 25xW MP, WD = 2 mm;
NA = 1.10
Filter Set
479/40 nm for mTFP1
534/30 nm for GFP
578/105 nm for TagRFP
Dichroic
DM495LP
DM560LP