454-Mizuta-PollenTubeGuidance/EV1A_PollenTube
Author reviewedPollen tube dynamics in the Arabidopsis wild-type pistil
Dataset ID
EV1A_PollenTube
Kind
Image data
Title
Pollen tube dynamics in the Arabidopsis wild-type pistil
Description
Z-series and time-lapse images of pollen tube expressing mTFP1 or TagRFP in wild type Arabidopsis.
Folder Name
EV1A_PollenTube
File Format
.nd2
File Size
1.8GB
File Separation Method
-
Figure Number
Fig1ABC
Dimensions
512x1485x10x2x65
X Scale
1 micrometer
Y Scale
1 micrometer
Z Scale
15 micrometer
T Scale
10 minutes
Channel
2 channel
Quantitative Data
-
Paper(s)
Biosample
Description
The dissected pistil of wild type Arabidopsis after hand pollination.
Organism
Arabidopsis thaliana (NCBITaxon:3702)
Strain
Cell
ovary (UBERON:0000992)
Cell line
-
MeSH
Intrinsic Variable
LAT52p::mTFP1 and LAT52p::TagRFP were expressed in the pollen tube.
Extrinsic Variable
-
Ontology
Anatomical Entity
pollen tube (EFO:0001004)
pistil (EFO:0001004)
UBERON
ovary (UBERON:0000992)
Biological Process
pollen tube guidance (GO:0010183)
Cellular Component
cytoplasm (GO:0005737)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transgenic / LAT52p::mTFP1
Gene
-
Protein / Probe
-
Method / Genotype
transgenic / LAT52p::TagRFP
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
mTFP1
TagRFP
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Nikon A1RMP
Module
-
Light Source
Spectra-Physics Mai Tai DeepSee
Detector
Nikon non-descanned GaAsP PMT
detectors
Objective
Nikon CFI75 Apo 25xW MP, WD = 2 mm;
NA = 1.10
Filter Set
479/40 nm for mTFP1
578/105 nm for TagRFP
Dichroic
DM495LP
DM560LP