451-Hitora-Neopetromin/Fig4_AF_LatB
Author reviewedZ-series images of GFP-fABD2-labeled actin filaments in tobacco BY-2 cells in the presence of latrunculin B
Project
Dataset ID
Fig4_AF_LatB
Kind
Image data
Title
Z-series images of GFP-fABD2-labeled actin filaments in tobacco BY-2 cells in the presence of latrunculin B
Description
Z-series images of GFP-fABD2-labeled actin filaments in tobacco BY-2 cells in the presence of latrunculin B. fABD2 is C-terminal half of AtFim1 (aa 325-687), which includes the second actin-binding domain.
Folder Name
Fig4_AF_LatB
File Format
.tif
File Size
114MB
File Separation Method
-
Figure Number
Fig4
Dimensions
731x957x82x1x1
X Scale
0.185 micrometer
Y Scale
0.185 micrometer
Z Scale
0.5 micrometer
T Scale
-
Channel
1 channel
Quantitative Data
-
Biosample
Description
Tobacco BY-2 cells stably expressing GFP-fABD2 that was treated with latrunculin B.
Organism
Nicotiana tabacum (NCBITaxon:4097)
Strain
-
Cell
-
Cell line
TBY-2 cell (BTO:0001972)
Intrinsic Variable
-
Extrinsic Variable
Latrunculin B (4 micromolar) treatment for 2 hours
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
negative regulation of actin filament polymerization (GO:0030837)
Cellular Component
actin cytoskeleton (GO:0015629)
Molecular Function
-
Genetics
Gene
-
Protein / Probe
-macromolecular probe,
fABD2 which binds to F-actin
Method / Genotype
transfection
Treatments
Reagent / Compound
latrunculin B
Concentration
4 micromolar
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
Green fluorescent protein from Aequorea
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
Instrument
Body
Olympus-Evident IX70
Module
-
Light Source
-
Detector
Prime
95B; Teledyne Photometrics, Tucson, AZ, USA
Objective
Olympus-Evident UPLSAP60XS2
Filter Set
-
Dichroic
-