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448-Temma-HeLaSPASIM/FigS12_twophoton_nuclei_rsG_WF thumbnail

448-Temma-HeLaSPASIM/FigS12_twophoton_nuclei_rsG_WF

Author reviewed
Fluorescence images of nuclei labeled by rsGamillus-S in HeLa cell spheroids in wide-field mode for two-photon activation
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Project
448-Temma-HeLaSPASIM
Dataset ID
FigS12_twophoton_nuclei_rsG_WF
Kind
Image data
Title
Fluorescence images of nuclei labeled by rsGamillus-S in HeLa cell spheroids in wide-field mode for two-photon activation
Description
Fluorescence images of nuclei in HeLa cell spheroids expressing rsGamillus-S-labeled histone H2B were obtained using wide-field (WF) fluorescence microscopy with two-photon activation by a Bessel sheet. rsGamillus-S is an acid-resistance photoswitchable fluorescent protein, which owns exceptionally high on-switching rate to show the nuclei more clearly. Two-photon activation using a scanned Bessel beam produces a wider FOV and a higher axial resolution.
Folder Name
FigS12_twophoton_nuclei_rsG_WF
File Format
tif
File Size
36MB
File Separation Method
-
Figure Number
FigureS12a
Dimensions
6141x6141x1x1x1
X Scale
141.8 micrometer
Y Scale
141.8 micrometer
Z Scale
-
T Scale
-
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
Cell spheroids consisted of HeLa cells stably expressing rsGamillus-S-H2B to label nuclei.
Organism
Homo sapiens
(NCBITaxon:9606)
Strain
-
Cell
-
Cell line
HeLa-S3 cell (CLO:0003699)
MeSH
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)
Intrinsic Variable
-
Extrinsic Variable
A living HeLa cell spheroid with histone H2B labeled by rsGamillus-S

Ontology

Anatomical Entity
-
UBERON
-
Biological Process
-
Cellular Component
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)
MeSH BP
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)
MeSH CC
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)
MeSH MF
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)

Genetics

Gene
-
Protein / Probe
H2B
Method / Genotype
electroporation

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
wide-field microscopy
Detection Method
wide-field detection
Visualization Method
rsGamillus-S
Illumination Method
wide-field illumination
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
-
MeSH
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)

Instrument

Body
-
Module
-
Light Source
405 nm wide-field illumination
Detector
Hamamatsu Photonics ORCA-Flash 4.0 v3
Objective
Nikon CFI75 Apo 25XC W, Special Optics 54-10-7
Filter Set
-
Dichroic
Di03-R488-t3-25x36, RazorEdge LongPass488, FF01-790/SP-25