SSBD:database

448-Temma-HeLaSPASIM/Fig3_twophoton_spheroid_SPASIM_shifted thumbnail

448-Temma-HeLaSPASIM/Fig3_twophoton_spheroid_SPASIM_shifted

Author reviewed
3D-rendered images of spheroid of living HeLa cells in SPA-SIM mode for two-photon activation
SSBD:OMERO.gallery (Project) SSBD:OMERO (Project) SSBD:OMERO.gallery (Dataset) SSBD:OMERO (Dataset)
SSBD:downloads
Project
448-Temma-HeLaSPASIM
Dataset ID
Fig3_twophoton_spheroid_SPASIM_shifted
Kind
Image data
Title
3D-rendered images of spheroid of living HeLa cells in SPA-SIM mode for two-photon activation
Description
3D-rendered images of living HeLa cell spheroids stably expressing rsGamillus-S-H2B to label nuclei were obtained using selective-plane-activation structured illumination microscopy (SPA-SIM) with two-photon activation and a scanned Bessel beam. rsGamillus-S is an acid-resistance photoswitchable fluorescent protein, which owns exceptionally high on-switching rate to show the nuclei more clearly. Two-photon activation using a scanned Bessel beam produces a wider FOV and a higher axial resolution.
Folder Name
Fig3_twophoton_spheroid_SPASIM_shifted
File Format
tif
File Size
2.8GB
File Separation Method
-
Figure Number
Figure3n
Dimensions
6141x6141x80x1x1
X Scale
141.8 micrometer
Y Scale
141.8 micrometer
Z Scale
125 nanometer
T Scale
-
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
Cell spheroids consisted of HeLa cells stably expressing rsGamillus-S-H2B to label nuclei.
Organism
Homo sapiens
(NCBITaxon:9606)
Strain
-
Cell
-
Cell line
HeLa-S3 cell (CLO:0003699)
MeSH
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)
Intrinsic Variable
-
Extrinsic Variable
A living HeLa cell spheroid with histone H2B labeled by rsGamillus-S

Ontology

Anatomical Entity
-
UBERON
-
Biological Process
-
Cellular Component
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)
MeSH BP
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)
MeSH CC
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)
MeSH MF
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)

Genetics

Gene
-
Protein / Probe
H2B
Method / Genotype
electroporation

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
structured illumination microscopy
(FBbi:00000332)
Detection Method
detection of photons
Visualization Method
rsGamillus-S
Illumination Method
light-sheet illumination
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
living cell
MeSH
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)

Instrument

Body
-
Module
-
Light Source
a scanned Bessel beam with a wavelength of 780 nm
Detector
Hamamatsu Photonics ORCA-Flash 4.0 v3
Objective
Nikon CFI75 Apo 25XC W, Special Optics 54-10-7
Filter Set
-
Dichroic
Di03-R488-t3-25x36, RazorEdge LongPass488, FF01-790/SP-25