SSBD:database

448-Temma-HeLaSPASIM/Fig3_twophoton_Lifeact_SPASIM_3D thumbnail

448-Temma-HeLaSPASIM/Fig3_twophoton_Lifeact_SPASIM_3D

Author reviewed
3D-rendered images of actin filaments of living HeLa cells in 3DSIM mode for two-photon activation
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Project
448-Temma-HeLaSPASIM
Dataset ID
Fig3_twophoton_Lifeact_SPASIM_3D
Kind
Image data
Title
3D-rendered images of actin filaments of living HeLa cells in 3DSIM mode for two-photon activation
Description
3D-rendered images of actin filaments in living HeLa cells visualized with rsGamillus-S were obtained using selective-plane-activation structured illumination microscopy (SPA-SIM) with two-photon activation and a scanned Bessel beam. rsGamillus-S is an acid-resistance photoswitchable fluorescent protein, which owns exceptionally high on-switching rate to show the nuclei more clearly. Two-photon activation using a scanned Bessel beam produces a wider FOV and a higher axial resolution.
Folder Name
Fig3_twophoton_Lifeact_SPASIM_3D
File Format
tif
File Size
1.8GB
File Separation Method
-
Figure Number
Figure3h
Dimensions
3069x3069x200x1x1
X Scale
70.9 micrometer
Y Scale
70.9 micrometer
Z Scale
125 nanometer
T Scale
-
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
HeLa cells were electroporated with rsGamillus-S plasmids to express fluorescent proteins. Actin filaments were visualized by rsGamillus-S.
Organism
Homo sapiens
(NCBITaxon:9606)
Strain
-
Cell
-
Cell line
HeLa-S3 cell (CLO:0003699)
MeSH
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Actin Cytoskeleton (D008841)
Intrinsic Variable
-
Extrinsic Variable
HeLa cells were electroporated with rsGamillus-S plasmids

Ontology

Anatomical Entity
-
UBERON
-
Biological Process
-
Cellular Component
actin filament (GO:0005884)
Molecular Function
-
MeSH Anatomy
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Actin Cytoskeleton (D008841)
MeSH BP
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Actin Cytoskeleton (D008841)
MeSH CC
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Actin Cytoskeleton (D008841)
MeSH MF
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Actin Cytoskeleton (D008841)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
electroporation

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
structured illumination microscopy
(FBbi:00000332)
Detection Method
detection of photons
Visualization Method
rsGamillus-S
Illumination Method
light-sheet illumination
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
living cell
MeSH
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Actin Cytoskeleton (D008841)

Instrument

Body
-
Module
-
Light Source
a scanned Bessel beam with a wavelength of 780 nm
Detector
Hamamatsu Photonics ORCA-Flash 4.0 v3
Objective
Nikon CFI75 Apo 25XC W, Special Optics 54-10-7
Filter Set
-
Dichroic
Di03-R488-t3-25x36, RazorEdge LongPass488