SSBD:database

Project

Dataset ID

Fig3_onephoton_mito_xy_SPASIM

Kind

Image data

Title

The x-y image of mitochondria of living HeLa cells in SPA-SIM mode for single-photon activation

Description

An x-y image of mitochondria in living HeLa cells visualized with Skylan-NS was captured using selective-plane-activation structured illumination microscopy (SPA-SIM) with single-photon activation. The x-y image shows the lateral distribution of fluorescence within mitochondria of living HeLa cells. Skylan-NS is a photoswitchable green fluorescent protein for improving super-resolution imaging for autophagosomes in living cells. A single-photon source is a light source that emits light as single particles or photons. The single-photon activation is single and with strong signal.

Folder Name

Fig3_onephoton_mito_xy_SPASIM

File Format

tif

File Size

18MB

File Separation Method

-

Figure Number

Figure3a

Dimensions

4359x4359x1x1x1

X Scale

100.7 micrometer

Y Scale

100.7 micrometer

Z Scale

-

T Scale

-

Channel

1 channel

Quantitative Data

-

Description

Mitochondria were visualized using mitochondria-targeted Skylan-NS in living HeLa cell spheroids.

Organism

Strain

-

Cell

-

Cell line

HeLa-S3 cell (CLO:0003699)

MeSH

Intrinsic Variable

-

Extrinsic Variable

HeLa cells were electroporated with Skylan-NS plasmids

Anatomical Entity

-

UBERON

-

Biological Process

-

Cellular Component

Molecular Function

-

MeSH Anatomy

MeSH BP

MeSH CC

MeSH MF

Imaging Method

Detection Method

detection of photons

Visualization Method

Skylan-NS

Illumination Method

light-sheet illumination

Source of Contrast

-

Imaged Parameter

-

Sample Preparation Method

living cell

MeSH

Body

-

Module

-

Light Source

405 nm light sheet illumination

Detector

Hamamatsu Photonics ORCA-Flash 4.0 v3

Objective

Nikon CFI75 Apo 25XC W, Special Optics 54-10-7

Filter Set

-

Dichroic

Di03-R488-t3-25x36, RazorEdge LongPass488