SSBD:database

Project

Dataset ID

Fig2_twophoton_H2B_spheroid_3DSIM

Kind

Image data

Title

Fluorescence images of spheroid of living HeLa cells labeled by rsGamillus-S in 3DSIM mode for two-photon activation

Description

Fluorescence images of histone H2B in the cell spheroids of living HeLa cells labeled by rsGamillus-S were obtained in three-dimensional structured illumination microscopy (3DSIM) mode using two-photon activation. The 3DSIM mode was difficult for imaging the internal structure of spheroid. rsGamillus-S is an acid-resistance photoswitchable fluorescent protein, which owns exceptionally high on-switching rate to show the nuclei more clearly. Two-photon activation using a scanned Bessel beam produces a wider FOV and a higher axial resolution.

Folder Name

Fig2_twophoton_H2B_spheroid_3DSIM

File Format

tif

File Size

36MB

File Separation Method

-

Figure Number

Figure2t

Dimensions

6141x6141x1x1x1

X Scale

35.4 micrometer

Y Scale

25400 micrometer

Z Scale

-

T Scale

-

Channel

1 channel

Quantitative Data

-

Description

Cell spheroids consisted of HeLa cells stably expressing rsGamillus-S-H2B to label nuclei.

Organism

Strain

-

Cell

-

Cell line

HeLa-S3 cell (CLO:0003699)

MeSH

Intrinsic Variable

-

Extrinsic Variable

A living HeLa cell spheroid with histone H2B labeled by rsGamillus-S

Anatomical Entity

-

UBERON

-

Biological Process

-

Cellular Component

Molecular Function

-

MeSH Anatomy

MeSH BP

MeSH CC

MeSH MF

Imaging Method

Detection Method

detection of photons

Visualization Method

rsGamillus-S

Illumination Method

wide-field illumination

Source of Contrast

-

Imaged Parameter

-

Sample Preparation Method

living cell

MeSH

Body

-

Module

-

Light Source

405 nm wide-field illumination

Detector

Hamamatsu Photonics ORCA-Flash 4.0 v3

Objective

Nikon CFI75 Apo 25XC W, Special Optics 54-10-7

Filter Set

-

Dichroic

Di03-R488-t3-25x36, RazorEdge LongPass488, FF01-790/SP-25