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448-Temma-HeLaSPASIM/Fig2_twophoton_H2B_nulceus_SPASIM thumbnail

448-Temma-HeLaSPASIM/Fig2_twophoton_H2B_nulceus_SPASIM

Author reviewed
Fluorescence images of histone H2B in the nucleus of living HeLa cells labeled by rsGamillus-S in SPA-SIM mode for two-photon activation
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Project
448-Temma-HeLaSPASIM
Dataset ID
Fig2_twophoton_H2B_nulceus_SPASIM
Kind
Image data
Title
Fluorescence images of histone H2B in the nucleus of living HeLa cells labeled by rsGamillus-S in SPA-SIM mode for two-photon activation
Description
Fluorescence images of histone H2B in the nucleus of living HeLa cells labeled by rsGamillus-S were obtained in selective-plane-activation structured illumination microscopy (SPA-SIM) mode using two-photon activation. SPA-SIM achieved the observation of the internal structures of the nucleus with high spatial resolution and image contrast. rsGamillus-S is an acid-resistance photoswitchable fluorescent protein, which owns exceptionally high on-switching rate to show the nuclei more clearly. Two-photon activation using a scanned Bessel beam produces a wider FOV and a higher axial resolution.
Folder Name
Fig2_twophoton_H2B_nulceus_SPASIM
File Format
tif
File Size
2.2MB
File Separation Method
-
Figure Number
Figure2p
Dimensions
1533x1533x1x1x1
X Scale
35.4 micrometer
Y Scale
35.4 micrometer
Z Scale
-
T Scale
-
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
Nuclei in living HeLa cell spheroids were visualized using rsGamillus-S-labeled histone H2B.
Organism
Homo sapiens
(NCBITaxon:9606)
Strain
-
Cell
-
Cell line
HeLa-S3 cell (CLO:0003699)
MeSH
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)
Intrinsic Variable
-
Extrinsic Variable
A living HeLa cell with histone H2B labeled by rsGamillus-S

Ontology

Anatomical Entity
-
UBERON
-
Biological Process
-
Cellular Component
nucleus (GO:0005634)
Molecular Function
-
MeSH Anatomy
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)
MeSH BP
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)
MeSH CC
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)
MeSH MF
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)

Genetics

Gene
-
Protein / Probe
H2B
Method / Genotype
electroporation

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
structured illumination microscopy
(FBbi:00000332)
Detection Method
detection of photons
Visualization Method
rsGamillus-S
Illumination Method
light-sheet illumination
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
living cell
MeSH
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Cell Nucleus (D002467)

Instrument

Body
-
Module
-
Light Source
a scanned Bessel beam with a wavelength of 780 nm
Detector
Hamamatsu Photonics ORCA-Flash 4.0 v3
Objective
Nikon CFI75 Apo 25XC W, Special Optics 54-10-7
Filter Set
-
Dichroic
Di03-R488-t3-25x36, RazorEdge LongPass488, FF01-790/SP-25