SSBD:database

Project

Dataset ID

Fig2_onephoton_mito_fixed_SPA3DSIM

Kind

Image data

Title

Fluorescence images of mitochondria in fixed HeLa cells in the imaging mode of SPA3DSIM

Description

The fluorescence images of mitochondria labeled with Skylan-NS in fixed HeLa cells were taken and reconstructed in the imaging mode of SPA-3DSIM. The optical sectioning capability in SPA-3DSIM mode had further improvements. The out-of-focus light in the image produced by the SPA-3DSIM was reduced more efficiently, while the practical spatial resolution was slightly inferior. Skylan-NS is a photoswitchable green fluorescent protein. CW laser is used for single-photon activation, which is more efficient and allows measurement with higher temporal resolution.

Folder Name

Fig2_onephoton_mito_fixed_SPA3DSIM

File Format

tif

File Size

9MB

File Separation Method

-

Figure Number

Figure2f

Dimensions

3069x3069x1x1x1

X Scale

70.9 micrometer

Y Scale

70.9 micrometer

Z Scale

-

T Scale

-

Channel

1 channel

Quantitative Data

-

Description

Mitochondria were visualized using mitochondria-targeted Skylan-NS in living HeLa cell spheroids.

Organism

Strain

-

Cell

-

Cell line

HeLa-S3 cell (CLO:0003699)

MeSH

Intrinsic Variable

-

Extrinsic Variable

HeLa cells were electroporated with Skylan-NS plasmids

Anatomical Entity

-

UBERON

-

Biological Process

-

Cellular Component

Molecular Function

-

MeSH Anatomy

MeSH BP

MeSH CC

MeSH MF

Imaging Method

Detection Method

detection of photons

Visualization Method

Skylan-NS

Illumination Method

light-sheet illumination

Source of Contrast

-

Imaged Parameter

-

Sample Preparation Method

fixed cell

MeSH

Body

-

Module

-

Light Source

405 nm light sheet illumination

Detector

Hamamatsu Photonics ORCA-Flash 4.0 v3

Objective

Nikon CFI75 Apo 25XC W, Special Optics 54-10-7

Filter Set

-

Dichroic

Di03-R488-t3-25x36, RazorEdge LongPass488