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448-Temma-HeLaSPASIM/Fig2_onephoton_mito_TL_SPIM thumbnail

448-Temma-HeLaSPASIM/Fig2_onephoton_mito_TL_SPIM

Author reviewed
Time-lapse images of mitochondria of living HeLa cells in SPIM mode for single-photon activation
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Project
448-Temma-HeLaSPASIM
Dataset ID
Fig2_onephoton_mito_TL_SPIM
Kind
Image data
Title
Time-lapse images of mitochondria of living HeLa cells in SPIM mode for single-photon activation
Description
The mitochondria labeled with Skylan-NS in living HeLa cells were obserbed in selective plane illumination microscopy (SPIM) mode using single-photon activation. SPIM visualizes mitochondrial fusion at a lower spatial resolution than that of SPA-SIM. Skylan-NS is a photoswitchable green fluorescent protein. CW laser is used for single-photon activation, which is more efficient and allows measurement with higher temporal resolution.
Folder Name
Fig2_onephoton_mito_TL_SPIM
File Format
tif
File Size
134MB
File Separation Method
-
Figure Number
Figure2h
Dimensions
1533x1533x60x1x1
X Scale
35.4 micrometer
Y Scale
35.4 micrometer
Z Scale
-
T Scale
4 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)

Biosample

Description
Mitochondria were visualized using mitochondria-targeted Skylan-NS in living HeLa cell spheroids.
Organism
Homo sapiens
(NCBITaxon:9606)
Strain
-
Cell
-
Cell line
HeLa-S3 cell (CLO:0003699)
MeSH
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Mitochondria (D008928)
Time-Lapse Imaging (D059008)
Intrinsic Variable
-
Extrinsic Variable
HeLa cells were electroporated with Skylan-NS plasmids

Ontology

Anatomical Entity
-
UBERON
-
Biological Process
-
Cellular Component
mitochondrion (GO:0005739)
Molecular Function
-
MeSH Anatomy
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Mitochondria (D008928)
Time-Lapse Imaging (D059008)
MeSH BP
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Mitochondria (D008928)
Time-Lapse Imaging (D059008)
MeSH CC
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Mitochondria (D008928)
Time-Lapse Imaging (D059008)
MeSH MF
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Mitochondria (D008928)
Time-Lapse Imaging (D059008)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
electroporation

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
structured illumination microscopy
(FBbi:00000332)
time lapse microscopy
(FBbi:00000249)
Detection Method
detection of photons
Visualization Method
Skylan-NS
Illumination Method
light-sheet illumination
Source of Contrast
-
Imaged Parameter
-
Sample Preparation Method
living cell
MeSH
HeLa Cells (D006367)
Microscopy, Fluorescence (D008856)
Mitochondria (D008928)
Time-Lapse Imaging (D059008)

Instrument

Body
-
Module
-
Light Source
405 nm light sheet illumination
Detector
Hamamatsu Photonics ORCA-Flash 4.0 v3
Objective
Nikon CFI75 Apo 25XC W, Special Optics 54-10-7
Filter Set
-
Dichroic
Di03-R488-t3-25x36, RazorEdge LongPass488